| Autor: |
Franco EPD; Laboratory of Multidisciplinary Research, São Francisco University (USF) , Bragança Paulista , Brazil., Contesini FJ; Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP) , Campinas , Brazil., Lima da Silva B; Laboratory of Multidisciplinary Research, São Francisco University (USF) , Bragança Paulista , Brazil., Alves de Piloto Fernandes AM; Laboratory of Multidisciplinary Research, São Francisco University (USF) , Bragança Paulista , Brazil., Wielewski Leme C; Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP) , Campinas , Brazil., Gonçalves Cirino JP; Laboratory of Multidisciplinary Research, São Francisco University (USF) , Bragança Paulista , Brazil., Bueno Campos PR; Laboratory of Multidisciplinary Research, São Francisco University (USF) , Bragança Paulista , Brazil., de Oliveira Carvalho P; Laboratory of Multidisciplinary Research, São Francisco University (USF) , Bragança Paulista , Brazil. |
| Abstrakt: |
Matricaria chamomilla L. contains antioxidant flavonoids that can have their bioactivity enhanced by enzymatic hydrolysis of specific glycosyl groups. This study implements an untargeted metabolomics approach based on ultra-performance liquid chromatography coupled with electrospray ionisation quadrupole time-of-flight mass spectrometry technique operating in MS E mode (UPLC-QTOF-MS E ) and spectrophotometric analysis of chamomile aqueous infusions, before and after hydrolysis by hesperidinase and β-galactosidase. Several phenolic compounds were altered in the enzymatically treated infusion, with the majority being flavonoid derivatives of apigenin, esculetin, and quercetin. Although enzymatically modifying the infusion only led to a small increase in antioxidant activity (DPPH • method), its inhibitory effect on pancreatic lipase was of particular interest. The enzymatically treated infusion exhibited a greater inhibitory effect (EC 50 of 35.6 µM) than unmodified infusion and kinetic analysis suggested mixed inhibition of pancreatic lipase. These results are of great relevance due to the potential of enzymatically treated functional foods in human health. |
