Development and validation of an LC-MS/MS method for profiling 39 urinary steroids (estrogens, androgens, corticoids, and progestins).

Autor: Son HH; Center for Chemical Analysis, Korea Research Institute of Chemical Technology (KRICT), Daejeon, Republic of Korea.; Department of Chemistry, Sungkyunkwan University, Suwon, Gyeonggi-Do, Republic of Korea., Yun WS; Department of Chemistry, Sungkyunkwan University, Suwon, Gyeonggi-Do, Republic of Korea., Cho SH; Center for Chemical Analysis, Korea Research Institute of Chemical Technology (KRICT), Daejeon, Republic of Korea.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2020 Feb; Vol. 34 (2), pp. e4723. Date of Electronic Publication: 2019 Dec 10.
DOI: 10.1002/bmc.4723
Abstrakt: Abnormal production or metabolism of steroid hormones is responsible for the development of endocrine diseases. Thus, accurate quantification of steroid hormones is needed for both research into clinical conditions and diagnostic and monitoring purposes. An improved analytical method for profiling 39 steroids in urine using LC-MS/MS was developed. As a pre-treatment procedure prior to LC-tandem mass spectrometry (LC-MS/MS) analysis, hydrolysis using β-glucuronidase and solid-phase extraction for purifying the samples were performed. Steroids were separated using Waters ACQUITY BEH C 18 column (2.1 × 100 mm, 1.7 μm) and a mobile phase consisting of eluent A (0.01% formic acid and 1 mm ammonium formate in water) and eluent B (0.01% formic acid and 1 mm ammonium formate in methanol) with a gradient program at a flow rate of 0.4 mL/min. Under the optimized method, the linearity of calibration curves was higher than 0.992. The limits of detection at signal-to-noise ratio of 3 were 0.03-90 ng/mL. The developed novel LC-MS/MS method can quantitatively profile 39 steroids in a single analytical run. Steroid profiling based on quantitative results could improve the diagnosis and monitoring of hormone-dependent diseases.
(© 2019 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
Externí odkaz: