| Autor: |
Makarenko MS; Department of Genetics, Southern Federal University, Rostov-on-Don 344006, Russia. mcmakarenko@yandex.ru.; The Institute for Information Transmission Problems, Moscow 127051, Russia. mcmakarenko@yandex.ru., Usatov AV; Department of Genetics, Southern Federal University, Rostov-on-Don 344006, Russia. usatova@sfedu.ru., Tatarinova TV; The Institute for Information Transmission Problems, Moscow 127051, Russia. ttatarinova@laverne.edu.; Department of Biology, University of La Verne, La Verne, CA 91750, USA. ttatarinova@laverne.edu.; Vavilov Institute of General Genetics, Moscow 119333, Russia. ttatarinova@laverne.edu.; School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk 660041, Russia. ttatarinova@laverne.edu., Azarin KV; Department of Genetics, Southern Federal University, Rostov-on-Don 344006, Russia. azarinkv@sfedu.ru., Logacheva MD; The Institute for Information Transmission Problems, Moscow 127051, Russia. m.logacheva@skoltech.ru.; Skolkovo Institute of Science and Technology, Moscow 121205, Russia. m.logacheva@skoltech.ru., Gavrilova VA; The N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Saint Petersburg 190121, Russia. v.gavrilova@vir.nw.ru., Kornienko IV; Department of Genetics, Southern Federal University, Rostov-on-Don 344006, Russia. ivkornienko@sfedu.ru.; Southern Scientific Center of the Russian Academy of Sciences, Rostov-on-Don 344006, Russia. ivkornienko@sfedu.ru., Horn R; Institute of Biological Sciences, Plant Genetics, University of Rostock, 18059 Rostock, Germany. renate.horn@uni-rostock.de. |
| Abstrakt: |
This study provides insights into the flexibility of the mitochondrial genome in sunflower ( Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CMS ANN2 mitochondrial DNA sequence revealed the following reorganization events: twelve rearrangements, seven insertions, and nine deletions. Comparisons of coding sequences from the male-sterile line with the male-fertile line identified a deletion of orf777 and seven new transcriptionally active open reading frames (ORFs): orf324 , orf327 , orf345 , orf558 , orf891 , orf933 , orf1197 . Three of these ORFs represent chimeric genes involving atp6 ( orf1197 ), cox2 ( orf558 ), and nad6 ( orf891 ). In addition, orf558 , orf891 , orf1197 , as well as orf933 , encode proteins containing membrane domain(s), making them the most likely candidate genes for CMS development in ANN2. Although the investigated CMS phenotype may be caused by simultaneous action of several candidate genes, we assume that orf1197 plays a major role in developing male sterility in ANN2. Comparative analysis of mitogenome organization in sunflower lines representing different CMS sources also allowed identification of reorganization hot spots in the mitochondrial genome of sunflower. |
