Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential.

Autor: Lam M; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Sanosaka T; Department of Physiology, Keio University School of Medicine, Tokyo, Japan., Lundin A; Astra Zeneca, Mölndal, Sweden., Imaizumi K; Department of Physiology, Keio University School of Medicine, Tokyo, Japan., Etal D; Astra Zeneca, Mölndal, Sweden., Karlsson FH; Astra Zeneca, Mölndal, Sweden., Clausen M; Astra Zeneca, Mölndal, Sweden., Cairns J; Astra Zeneca, Mölndal, Sweden., Hicks R; Astra Zeneca, Mölndal, Sweden., Kohyama J; Department of Physiology, Keio University School of Medicine, Tokyo, Japan., Kele M; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Okano H; Department of Physiology, Keio University School of Medicine, Tokyo, Japan., Falk A; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Jazyk: angličtina
Zdroj: Genes to cells : devoted to molecular & cellular mechanisms [Genes Cells] 2019 Dec; Vol. 24 (12), pp. 836-847. Date of Electronic Publication: 2019 Nov 17.
DOI: 10.1111/gtc.12731
Abstrakt: We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.
(© 2019 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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