Dissecting the molecular evolution of fluoroquinolone-resistant Shigella sonnei.

Autor:	Chung The H; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Boinett C; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Oxford University, Oxford, UK., Pham Thanh D; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Jenkins C; Gastrointestinal Bacterial Reference Unit, National Infection Service, Public Health England, London, UK., Weill FX; Institut Pasteur, Unité des Bactéries Pathogènes Entériques, Paris, France., Howden BP; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia., Valcanis M; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia., De Lappe N; National Salmonella, Shigella, and Listeria monocytogenes Reference Laboratory, University Hospital Galway, Galway, Ireland., Cormican M; School of Medicine, National University of Ireland Galway, Galway, Ireland., Wangchuk S; Public Health Laboratory, Department of Public Health, Ministry of Health, Royal Government of Bhutan, Thimphu, Bhutan., Bodhidatta L; Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand., Mason CJ; Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand., Nguyen TNT; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Ha Thanh T; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Voong VP; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Duong VT; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam., Nguyen PHL; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam., Turner P; Centre for Tropical Medicine and Global Health, Oxford University, Oxford, UK.; Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia., Wick R; Department of Infectious Diseases, Central Clinical School, Monash University, Melbourne, VIC, 3004, Australia., Ceyssens PJ; Unit Bacterial Diseases, Sciensano, Brussels, Belgium., Thwaites G; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Oxford University, Oxford, UK., Holt KE; Department of Infectious Diseases, Central Clinical School, Monash University, Melbourne, VIC, 3004, Australia.; London School of Hygiene and Tropical Medicine, London, UK., Thomson NR; London School of Hygiene and Tropical Medicine, London, UK.; The Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK., Rabaa MA; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. mrabaa@oucru.org.; Centre for Tropical Medicine and Global Health, Oxford University, Oxford, UK. mrabaa@oucru.org., Baker S; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam.; Centre for Tropical Medicine and Global Health, Oxford University, Oxford, UK.; Cambridge Institute of Therapeutic Immunology and Infectious Disease, The Department of Medicine, University of Cambridge, Cambridge, UK.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2019 Oct 23; Vol. 10 (1), pp. 4828. Date of Electronic Publication: 2019 Oct 23.
DOI:	10.1038/s41467-019-12823-0
Abstrakt:	Shigella sonnei increasingly dominates the international epidemiological landscape of shigellosis. Treatment options for S. sonnei are dwindling due to resistance to several key antimicrobials, including the fluoroquinolones. Here we analyse nearly 400 S. sonnei whole genome sequences from both endemic and non-endemic regions to delineate the evolutionary history of the recently emergent fluoroquinolone-resistant S. sonnei. We reaffirm that extant resistant organisms belong to a single clonal expansion event. Our results indicate that sequential accumulation of defining mutations (gyrA-S83L, parC-S80I, and gyrA-D87G) led to the emergence of the fluoroquinolone-resistant S. sonnei population around 2007 in South Asia. This clone was then transmitted globally, resulting in establishments in Southeast Asia and Europe. Mutation analysis suggests that the clone became dominant through enhanced adaptation to oxidative stress. Experimental evolution reveals that under fluoroquinolone exposure in vitro, resistant S. sonnei develops further intolerance to the antimicrobial while the susceptible counterpart fails to attain complete resistance.
Databáze:	MEDLINE
Externí odkaz:	Full text from SpringerLink