Oily core/amphiphilic polymer shell nanocapsules change the intracellular fate of doxorubicin in breast cancer cells.

Autor: Coelho JM; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br and Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Camargo NS; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br and Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Ganassin R; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br and Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Rocha MCO; Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Merker C; Institute of Medical Physics & Biophysics, Leipzig University, Leipzig 04107, Germany., Böttner J; Institute of Medical Physics & Biophysics, Leipzig University, Leipzig 04107, Germany., Estrela-Lopis I; Institute of Medical Physics & Biophysics, Leipzig University, Leipzig 04107, Germany., Py-Daniel KR; Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Jardim KV; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br., Sousa MH; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br., Ombredane AS; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br and Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil and Laboratory of Bioactive Compounds and Nanobiotechnology, Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil., Joanitti GA; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br and Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil and Laboratory of Bioactive Compounds and Nanobiotechnology, Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil., Silva RC; Institute of Metrology, Quality and Technology (Inmetro), Rio de Janeiro, 20250-020, Brazil., Azevedo RB; Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Longo JPF; Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil., Muehlmann LA; Faculty of Ceilandia, University of Brasilia, Brasilia 72220-900, Brazil. luisalex@unb.br.
Jazyk: angličtina
Zdroj: Journal of materials chemistry. B [J Mater Chem B] 2019 Oct 23; Vol. 7 (41), pp. 6390-6398.
DOI: 10.1039/c9tb00587k
Abstrakt: The aim of this work was to develop and test the in vitro biological activity of nanocapsules loaded with a doxorubicin (DOX) free base dissolved in a core of castor oil shelled by poly(methyl vinyl ether-co-maleic anhydride) conjugated to n-octadecylamine residues. This system was stable and monodisperse, with a hydrodynamic diameter of about 300 nm. These nanocapsules changed the intracellular distribution of DOX, from the nuclei to the cytoplasm, and exhibited higher toxicity towards cancer cells - 4T1 and MCF-7 - and significantly lower toxicity towards normal cells - NIH-3T3 and MCF-10A - in vitro. In conclusion, these nanocapsules are suitable DOX carriers, which remain to be studied in in vivo tumor models.
Databáze: MEDLINE