Benefits of non-invasive methods compared to telemetry for distress analysis in a murine model of pancreatic cancer.

Autor:	Kumstel S; Rudolf-Zenker-Institute of Experimental Surgery, University Medical Center, 18057 Rostock, Germany., Vasudevan P; Department of Cardiac Surgery, School of Medicine, University of Rostock, 18057 Rostock, Germany.; Department of Life, Light and Matter, University of Rostock, 18059 Rostock, Germany., Palme R; Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine, A-1210 Vienna, Austria., Zhang X; Rudolf-Zenker-Institute of Experimental Surgery, University Medical Center, 18057 Rostock, Germany., Wendt EHU; Rudolf-Zenker-Institute of Experimental Surgery, University Medical Center, 18057 Rostock, Germany., David R; Department of Cardiac Surgery, School of Medicine, University of Rostock, 18057 Rostock, Germany.; Department of Life, Light and Matter, University of Rostock, 18059 Rostock, Germany., Vollmar B; Rudolf-Zenker-Institute of Experimental Surgery, University Medical Center, 18057 Rostock, Germany., Zechner D; Rudolf-Zenker-Institute of Experimental Surgery, University Medical Center, 18057 Rostock, Germany.
Jazyk:	angličtina
Zdroj:	Journal of advanced research [J Adv Res] 2019 Sep 14; Vol. 21, pp. 35-47. Date of Electronic Publication: 2019 Sep 14 (Print Publication: 2020).
DOI:	10.1016/j.jare.2019.09.002
Abstrakt:	Prospective severity assessment is legally required in many countries to ensure high-quality research along with high welfare standards for laboratory animals. Mice and rats, the most common laboratory species, are prey animals that usually suppress signs of pain and suffering. Therefore, highly sensitive readout parameters are necessary to adequately quantify distress. The present study compared the performance of different non-invasive methods in determining animal distress, such as measuring body weight, distress score, faecal corticosterone metabolites, burrowing, and nesting behaviour, with continuous monitoring of heart rate, body temperature and activity by telemetry. The distress caused by two surgical interventions was compared and the burden caused by tumour growth was described. Transmitter implantation caused higher distress than laparotomy plus carcinoma cell injection into the pancreas. Surprisingly, no significant increase in distress was observed during tumour growth. The receiver operating characteristic curve analysis revealed that some non-invasive distress-parameters, i.e., distress-score and burrowing activity, exhibited slightly better performance to quantify distress than the most suitable parameters measured by telemetry. Due to the high burden caused by the implantation of the telemetric device, the use of non-invasive methods to assess distress in laboratory animals after surgical interventions should be favoured in future studies. Competing Interests: The authors declare no conflict of interest. (© 2019 THE AUTHORS. Published by Elsevier BV on behalf of Cairo University.)
Databáze:	MEDLINE
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