Exploring a Pool-seq-only approach for gaining population genomic insights in nonmodel species.

Autor: Kurland S; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden., Wheat CW; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden., de la Paz Celorio Mancera M; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden., Kutschera VE; Science for Life Laboratory and Department for Biochemistry and Biophysics Stockholm University Solna Sweden., Hill J; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden., Andersson A; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden., Rubin CJ; Department of Medical Biochemistry and Microbiology Uppsala University Uppsala Sweden., Andersson L; Department of Medical Biochemistry and Microbiology Uppsala University Uppsala Sweden.; Department of Animal Breeding and Genetics Swedish University of Agricultural Sciences Uppsala Sweden.; Department of Veterinary Integrative Biosciences Texas A&M University College Station TX USA., Ryman N; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden., Laikre L; Division of Population Genetics Department of Zoology Stockholm University Stockholm Sweden.
Jazyk: angličtina
Zdroj: Ecology and evolution [Ecol Evol] 2019 Sep 26; Vol. 9 (19), pp. 11448-11463. Date of Electronic Publication: 2019 Sep 26 (Print Publication: 2019).
DOI: 10.1002/ece3.5646
Abstrakt: Developing genomic insights is challenging in nonmodel species for which resources are often scarce and prohibitively costly. Here, we explore the potential of a recently established approach using Pool-seq data to generate a de novo genome assembly for mining exons, upon which Pool-seq data are used to estimate population divergence and diversity. We do this for two pairs of sympatric populations of brown trout ( Salmo trutta ): one naturally sympatric set of populations and another pair of populations introduced to a common environment. We validate our approach by comparing the results to those from markers previously used to describe the populations (allozymes and individual-based single nucleotide polymorphisms [SNPs]) and from mapping the Pool-seq data to a reference genome of the closely related Atlantic salmon ( Salmo salar ). We find that genomic differentiation ( F ST ) between the two introduced populations exceeds that of the naturally sympatric populations ( F ST  = 0.13 and 0.03 between the introduced and the naturally sympatric populations, respectively), in concordance with estimates from the previously used SNPs. The same level of population divergence is found for the two genome assemblies, but estimates of average nucleotide diversity differ ( π ¯  ≈ 0.002 and π ¯  ≈ 0.001 when mapping to S. trutta and S. salar , respectively), although the relationships between population values are largely consistent. This discrepancy might be attributed to biases when mapping to a haploid condensed assembly made of highly fragmented read data compared to using a high-quality reference assembly from a divergent species. We conclude that the Pool-seq-only approach can be suitable for detecting and quantifying genome-wide population differentiation, and for comparing genomic diversity in populations of nonmodel species where reference genomes are lacking.
Competing Interests: None declared.
(© 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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