| Autor: |
Wan Y; Center for Craniofacial Regeneration, Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA., White C; Center for Craniofacial Regeneration, Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA., Robert N; Center for Craniofacial Regeneration, Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA., Rogers MB; Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA., Szabo-Rogers HL; Center for Craniofacial Regeneration, Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA.; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA.; McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA. |
| Abstrakt: |
Optic development involves sequential interactions between several different tissue types, including the overlying ectoderm, adjacent mesoderm, and neural crest mesenchyme and the neuroectoderm. In an ongoing expression screen, we identified that Tfap2β , Casq2 , Penk , Zic1 , and Zic3 are expressed in unique cell types in and around the developing eye. Tfap2β , Zic1 , and Zic3 are transcription factors, Casq2 is a calcium binding protein and Penk is a neurotransmitter. Tfap2β , Zic1 , and Zic3 have reported roles in brain and craniofacial development, while Casq2 and Penk have unknown roles. These five genes are expressed in the major tissue types in the eye, including the muscles, nerves, cornea, and sclera. Penk expression is found in the sclera and perichondrium. At E12.5 and E15.5, the extra-ocular muscles express Casq2 , the entire neural retina expresses Zic1 , and Zic3 is expressed in the optic disk and lip of the optic cup. The expression of Tfap2β expanded from corneal epithelium to the neural retina between E12.5 to E15.5. These genes are expressed in similar domains as Hedgehog ( Gli1 , and Ptch1 ) and the Wnt ( Lef1 ) pathways. The expression patterns of these five genes warrant further study to determine their role in eye morphogenesis. |
