LncRNA AC009022.1 enhances colorectal cancer cells proliferation, migration, and invasion by promoting ACTR3B expression via suppressing miR-497-5p.
Autor: | Yu C; Department of Anus & Intestine Surgery, Weifang People's Hospital, Weifang, China., Zhang F; Department of Anus & Intestine Surgery, Weifang People's Hospital, Weifang, China. |
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Jazyk: | angličtina |
Zdroj: | Journal of cellular biochemistry [J Cell Biochem] 2020 Feb; Vol. 121 (2), pp. 1934-1944. Date of Electronic Publication: 2019 Oct 21. |
DOI: | 10.1002/jcb.29428 |
Abstrakt: | Purpose: Long noncoding RNAs (lncRNAs) play an indispensable role in cancer progression. We aim at exploring the effect of AC009022.1 on colorectal cancer (CRC) development in this paper. Methods: CRC tissues and matched normal tissues of 68 CRC patients were collected. HCT-116 and SW620 cells were transfected and grouped. The cell counting kit-8 assay, cell scratch test, and Transwell experiment were performed to sequentially detect cells proliferation, migration, and invasion. In vivo experiments were conducted. The Luciferase reporter gene assay was used. Gene expression was detected by quantitative reverse transcription polymerase chain reaction and Western blot analysis. Results: AC009022.1 expression was abnormally elevated in CRC tissues and cells. High AC009022.1 expression in CRC patients was significantly associated with poor prognosis. Compared with the siNC group, HCT-116 and SW620 cells of siAC009022.1 group exhibited much lower OD Conclusions: AC009022.1 enhanced CRC cell proliferation, migration, and invasion by promoting ACTR3B expression via suppressing miR-497-5p. (© 2019 Wiley Periodicals, Inc.) |
Databáze: | MEDLINE |
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