Occurrence and molecular characterization of betanodaviruses in fish and invertebrates of the Greek territorial waters.

Autor: Bitchava K; Laboratory of Fish, Shellfish & Crustacean Diseases, Veterinary Research Institute of Thessaloniki ELGO-DEMETER, Thessaloniki, Greece.; Laboratory of Ichthyology and Aquatic Animal Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece., Chassalevris T; Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece., Lampou E; Laboratory of Ichthyology and Aquatic Animal Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece., Athanassopoulou F; Laboratory of Ichthyology and Aquatic Animal Medicine, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece., Economou V; Laboratory of Hygiene of Food of Animal Origin - Veterinary Public Health, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece., Dovas CI; Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2019 Dec; Vol. 42 (12), pp. 1773-1783. Date of Electronic Publication: 2019 Oct 21.
DOI: 10.1111/jfd.13098
Abstrakt: Betanodaviruses are small ssRNA viruses that cause viral encephalopathy and retinopathy, a severe neuropathological infectious disease in marine fish species worldwide. In the present study, the occurrence of betanodaviruses was investigated in wild and cultured populations of fishes and invertebrates of the Greek territorial waters. Betanodaviruses were detected in 35 species belonging to 21 families and 12 orders. To our knowledge, 23 of those are reported for the first time in Greek waters, while 11 of them are reported for the first time globally. The positive samples were subjected to sequencing and phylogenetic analysis of partial segments of RNA1 and RNA2 genes. Almost all the viruses circulating in Greece fell within RGNNV genotype, while reassortant viruses were detected in three samples, namely two inter-RGNNV and one RGNNV/SJNNV. A novel unclassified Betanodavirus sequence was also identified. Most of the Greek sequence types have a restricted geographic distribution except for two RNA1 and one RNA2 sequence types that are widespread throughout the Mediterranean basin. The results of this study indicate the range of reservoirs/hosts of betanodaviruses and also their wide spread in the Greek territorial waters and reinforce the hypothesis that wild fish species transmit the virus to cultured ones and vice versa.
(© 2019 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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