AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD.

Autor:	Prain K; Pathology Queensland Central Laboratory, Division of Immunology, Royal Brisbane and Women's Hospital, Herston, QLD, Australia., Woodhall M; Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom., Vincent A; Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom., Ramanathan S; Brain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, Australia.; Brain and Mind Centre, University of Sydney, Camperdown, NSW, Australia., Barnett MH; Brain and Mind Centre, University of Sydney, Camperdown, NSW, Australia., Bundell CS; School of Biomedical Science, Medicine, University of Western Australia, Nedlands, WA, Australia.; PathWest Laboratory Medicine, Department of Immunology, QEII Medical Centre, Nedlands, WA, Australia., Parratt JDE; Brain and Mind Centre, University of Sydney, Camperdown, NSW, Australia.; Department of Neurology, Royal North Shore Hospital, Sydney, NSW, Australia., Silvestrini RA; Brain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, Australia., Bukhari W; School of Medicine, Gold Coast Campus, Griffith University, Southport, QLD, Australia.; Department of Neurology, Gold Coast University Hospital, Southport, QLD, Australia., Brilot F; Brain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, Australia.; Brain and Mind Centre, University of Sydney, Camperdown, NSW, Australia., Waters P; Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom., Broadley SA; School of Medicine, Gold Coast Campus, Griffith University, Southport, QLD, Australia.; Department of Neurology, Gold Coast University Hospital, Southport, QLD, Australia.
Jazyk:	angličtina
Zdroj:	Frontiers in neurology [Front Neurol] 2019 Oct 04; Vol. 10, pp. 1028. Date of Electronic Publication: 2019 Oct 04 (Print Publication: 2019).
DOI:	10.3389/fneur.2019.01028
Abstrakt:	We have compared five different assays for antibodies to aquaporin-4 in 181 cases of suspected Neuromyelitis optica spectrum disorders (NMOSD) and 253 controls to assess their relative utility. As part of a clinically-based survey of NMOSD in Australia and New Zealand, cases of suspected NMOSD were referred from 23 centers. Clinical details and magnetic imaging were reviewed and used to apply the 2015 IPND diagnostic criteria. In addition, 101 age- and sex-matched patients with multiple sclerosis were referred. Other inflammatory disease ( n = 49) and healthy controls ( n = 103) were also recruited. Samples from all participants were tested using tissue-based indirect immunofluorescence assays and a subset were tested using four additional ELISA and cell-based assays. Antibodies to myelin oligodendrocyte glycoprotein (MOG) were also assayed. All aquaporin-4 antibody assays proved to be highly specific. Sensitivities ranged from 60 to 94%, with cell-based assays having the highest sensitivity. Antibodies to MOG were detected in 8/79 (10%) of the residual suspected cases of NMOSD. Under the 2015 IPND diagnostic criteria for NMOSD, cell-based assays for aquaporin-4 are sensitive and highly specific, performing better than tissue-based and ELISA assays. A fixed cell-based assay showed near-identical results to a live-cell based assay. Antibodies to MOG account for only a small number of suspected cases. (Copyright © 2019 Prain, Woodhall, Vincent, Ramanathan, Barnett, Bundell, Parratt, Silvestrini, Bukhari, The Australian and New Zealand NMO Collaboration, Brilot, Waters and Broadley.)
Databáze:	MEDLINE
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