Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

Autor:	Cossarizza A; Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy., Chang HD; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Radbruch A; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Acs A; Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany., Adam D; Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany., Adam-Klages S; Institut für Transfusionsmedizin, Universitätsklinik Schleswig-Holstein, Kiel, Germany., Agace WW; Mucosal Immunology group, Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.; Immunology Section, Lund University, Lund, Sweden., Aghaeepour N; Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences; and Pediatrics, Stanford University, Stanford, CA, USA., Akdis M; Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland., Allez M; Université de Paris, Institut de Recherche Saint-Louis, INSERM U1160, and Gastroenterology Department, Hôpital Saint-Louis - APHP, Paris, France., Almeida LN; Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany., Alvisi G; Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy., Anderson G; University of Birmingham, Birmingham, UK., Andrä I; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany., Annunziato F; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Anselmo A; Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy., Bacher P; Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.; Institut für Klinische Molekularbiologie, Christian-Albrechts Universität zu Kiel, Germany., Baldari CT; Department of Life Sciences, University of Siena, Siena, Italy., Bari S; Division of Medical Sciences, National Cancer Centre Singapore, Singapore.; Cancer & Stem Cell Biology, Duke-NUS Medical School, Singapore., Barnaba V; Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy.; Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.; Istituto Pasteur - Fondazione Cenci Bolognetti, Rome, Italy., Barros-Martins J; Institute of Immunology, Hannover Medical School, Hannover, Germany., Battistini L; IRCCS Fondazione Santa Lucia, Rome, Italy., Bauer W; Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria., Baumgart S; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Baumgarth N; Center for Comparative Medicine & Dept. Pathology, Microbiology & Immunology, University of California, Davis, CA, USA., Baumjohann D; Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany., Baying B; Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Bebawy M; Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, Sydney, NSW, Australia., Becher B; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.; Comprehensive Cancer Center Zurich, Switzerland., Beisker W; Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health, München, Germany., Benes V; Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Beyaert R; Department of Biomedical Molecular Biology, Center for Inflammation Research, Ghent University - VIB, Ghent, Belgium., Blanco A; Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland., Boardman DA; Department of Surgery, The University of British Columbia, Vancouver, Canada.; BC Children's Hospital Research Institute, Vancouver, Canada., Bogdan C; Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Erlangen, Germany.; Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Medical Immunology Campus Erlangen, Erlangen, Germany., Borger JG; Department of Immunology and Pathology, Monash University, Melbourne, Victoria, Australia., Borsellino G; Neuroimmunology and Flow Cytometry Units, Fondazione Santa Lucia IRCCS, Rome, Italy., Boulais PE; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, New York, USA., Bradford JA; Thermo Fisher Scientific, Eugene, Oregon, USA., Brenner D; Luxembourg Institute of Health, Department of Infection and Immunity, Experimental and Molecular Immunology, Esch-sur-Alzette, Luxembourg.; Odense University Hospital, Odense Research Center for Anaphylaxis, University of Southern Denmark, Department of Dermatology and Allergy Center, Odense, Denmark.; Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux, Luxembourg., Brinkman RR; Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada.; Terry Fox Laboratory, BC Cancer, Vancouver, BC, Canada., Brooks AES; University of Auckland, School of Biological Sciences, Maurice Wilkins Center, Auckland, New Zealand., Busch DH; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.; German Center for Infection Research (DZIF), Munich, Germany.; Focus Group ''Clinical Cell Processing and Purification', Institute for Advanced Study, Technische Universität München, Munich, Germany., Büscher M; Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Bushnell TP; Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester, NY, USA., Calzetti F; University of Verona, Department of Medicine, Section of General Pathology, Verona, Italy., Cameron G; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., Cammarata I; Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy., Cao X; National Key Laboratory of Medical Immunology, Nankai University, Tianjin, China., Cardell SL; Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden., Casola S; The FIRC Institute of Molecular Oncology (FOM), Milan, Italy., Cassatella MA; University of Verona, Department of Medicine, Section of General Pathology, Verona, Italy., Cavani A; National Institute for Health, Migration and Poverty (INMP), Rome, Italy., Celada A; Macrophage Biology Group, School of Biology, University of Barcelona, Barcelona, Spain., Chatenoud L; Université Paris Descartes, Institut National de la Santé et de la Recherche Médicale, Paris, France., Chattopadhyay PK; Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center, New York, USA., Chow S; Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada., Christakou E; Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.; National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health Service, Foundation Trust and King's College London, UK., Čičin-Šain L; Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Clerici M; IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.; Department of Physiopathology and Transplants, University of Milan, Milan, Italy.; Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy., Colombo FS; Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy., Cook L; BC Children's Hospital Research Institute, Vancouver, Canada.; Department of Medicine, The University of British Columbia, Vancouver, Canada., Cooke A; Department of Pathology, University of Cambridge, Cambridge, UK., Cooper AM; Department of Respiratory Sciences, University of Leicester, Leicester, UK., Corbett AJ; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., Cosma A; National Cytometry Platform, Luxembourg Institute of Health, Department of Infection and Immunity, Esch-sur-Alzette, Luxembourg., Cosmi L; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Coulie PG; de Duve Institute, Université catholique de Louvain, Brussels, Belgium., Cumano A; Unit Lymphopoiesis, Department of Immunology, Institut Pasteur, Paris, France., Cvetkovic L; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Dang VD; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Dang-Heine C; Clinical Research Unit, Berlin Institute of Health (BIH), Charite Universitätsmedizin Berlin, Berlin, Germany., Davey MS; Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, Victoria, Australia., Davies D; Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, UK., De Biasi S; Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy., Del Zotto G; IRCCS Istituto Giannina Gaslini, Genova, Italy., Dela Cruz GV; Novo Nordisk Foundation Center for Stem Cell Biology - DanStem, University of Copenhagen, Copenhagen, Denmark., Delacher M; Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.; Chair for Immunology, University Regensburg, Germany., Della Bella S; Department of Medical Biotechnologies and Translational Medicine, University of Milan, Milan, Italy., Dellabona P; Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy., Deniz G; Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey., Dessing M; Sony Europe Ltd, Weybridge, UK., Di Santo JP; Innate Immunty Unit, Department of Immunology, Institut Pasteur, Paris, France.; Institut Pasteur, Inserm U1223, Paris, France., Diefenbach A; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Charité - Universitätsmedizin Berlin, Laboratory of Innate Immunity, Department of Microbiology, Infectious Diseases and Immunology, Berlin, Germany.; Berlin Institute of Health (BIH), Berlin, Germany., Dieli F; University of Palermo, Central Laboratory of Advanced Diagnosis and Biomedical Research, Department of Biomedicine, Neurosciences and Advanced Diagnostics, Palermo, Italy., Dolf A; Flow Cytometry Core Facility, Institute of Experimental Immunology, University of Bonn, Bonn, Germany., Dörner T; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Dept. Medicine/Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Germany., Dress RJ; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore., Dudziak D; Department of Dermatology, Laboratory of Dendritic Cell Biology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), University Hospital Erlangen, Erlangen, Germany., Dustin M; Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK., Dutertre CA; Program in Emerging Infectious Disease, Duke-NUS Medical School, Singapore.; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore., Ebner F; Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany., Eckle SBG; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., Edinger M; Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.; Department of Internal Medicine III, University Hospital Regensburg, Germany., Eede P; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany., Ehrhardt GRA; Department of Immunology, University of Toronto, Toronto, ON, Canada., Eich M; Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany., Engel P; University of Barcelona, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences, Barcelona, Spain., Engelhardt B; Theodor Kocher Institute, University of Bern, Bern, Switzerland., Erdei A; Department of Immunology, University L. Eotvos, Budapest, Hungary., Esser C; Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany., Everts B; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Evrard M; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore., Falk CS; Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany., Fehniger TA; Division of Oncology, Washington University School of Medicine, St. Louis, MO, USA., Felipo-Benavent M; Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Principe Felipe Research Center, Valencia, Spain., Ferry H; Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Feuerer M; Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.; Chair for Immunology, University Regensburg, Germany., Filby A; The Flow Cytometry Core Facility, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK., Filkor K; MDQuest Ltd., Szeged, Hungary., Fillatreau S; Institut Necker-Enfants Malades, Université Paris Descartes Sorbonne Paris Cité, Faculté de Médecine, AP-HP, Hôpital Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Paris, France., Follo M; Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Universitaetsklinikum Freiburg, Lighthouse Core Facility, Zentrum für Translationale Zellforschung, Klinik für Innere Medizin I, Freiburg, Germany., Förster I; Immunology and Environment, LIMES Institute, University of Bonn, Bonn, Germany., Foster J; Owl Biomedical Inc., Santa Barbara, USA., Foulds GA; John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK., Frehse B; Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany., Frenette PS; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, New York, USA.; Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA., Frischbutter S; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venereology and Allergology., Fritzsche W; Nanobiophotonics Department, Leibniz Institute of Photonic Technology (IPHT), Jena, Germany., Galbraith DW; School of Plant Sciences and Bio5 Institute, University of Arizona, Tucson, USA.; Honorary Dean of Life Sciences, Henan University, Kaifeng, China., Gangaev A; Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands., Garbi N; Institute of Experimental Immunology, University of Bonn, Germany., Gaudilliere B; Stanford Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, CA, USA., Gazzinelli RT; Fundação Oswaldo Cruz - Minas, Laboratory of Immunopatology, Belo Horizonte, MG, Brazil.; Department of Mecicine, University of Massachusetts Medical School, Worcester, MA, USA., Geginat J; INGM - Fondazione Istituto Nazionale di Genetica Molecolare 'Ronmeo ed Enrica Invernizzi', Milan, Italy., Gerner W; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.; Christian Doppler Laboratory for Optimized Prediction of Vaccination Success in Pigs, Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria., Gherardin NA; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., Ghoreschi K; Department of Dermatology, Venereology and Allergology, Charité - Universitätsmedizin Berlin, Berlin, Germany., Gibellini L; Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy., Ginhoux F; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.; Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore.; Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China., Goda K; Department of Bioengineering, University of California, Los Angeles, California, USA.; Department of Chemistry, University of Tokyo, Tokyo, Japan.; Institute of Technological Sciences, Wuhan University, Wuhan, China., Godfrey DI; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., Goettlinger C; Institut für Genetik, Universität zu Köln, Köln, Germany., González-Navajas JM; Alicante Institute for Health and Biomedical Research (ISABIAL), Alicante, Spain.; Networked Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd), Madrid, Spain., Goodyear CS; Institute of Infection Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, UK., Gori A; Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, University of Milan., Grogan JL; Cancer Immunology Research, Genentech, South San Francisco, CA, USA., Grummitt D; Owl Biomedical Inc., Santa Barbara, USA., Grützkau A; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Haftmann C; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland., Hahn J; Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen., Hammad H; Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Zwijnaarde, Belgium., Hämmerling G; German Cancer Research Center (DKFZ), Heidelberg, Germany., Hansmann L; Berlin Institute of Health (BIH), Berlin, Germany.; German Cancer Consortium (DKTK), partner site Berlin, Berlin, Germany.; Department of Hematology, Oncology, and Tumor Immunology, Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany., Hansson G; Department of Medicine and Center for Molecular Medicine at Karolinska University Hospital, Solna, Sweden., Harpur CM; Murdoch Children's Research Institute, Parkville, Victoria, Australia., Hartmann S; Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany., Hauser A; Department of Internal Medicine III, University Hospital Regensburg, Germany., Hauser AE; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin.; Department of Rheumatology and Clinical Immunology, Berlin Institute of Health, Berlin, Germany., Haviland DL; Flow Cytometry, Houston Methodist Hospital Research Institute, Houston, TX, USA., Hedley D; Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada., Hernández DC; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Charité - Universitätsmedizin Berlin, Medical Department I, Division of Gastroenterology, Infectiology and Rheumatology, Berlin, Germany., Herrera G; Cytometry Service, Incliva Foundation. Clinic Hospital and Faculty of Medicine, University of Valencia, Valencia, Spain., Herrmann M; Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen., Hess C; Immunobiology Laboratory, Department of Biomedicine, University and University Hospital Basel, Basel, Switzerland.; Cambridge Institute of Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK., Höfer T; German Cancer Research Center (DKFZ), Division of Theoretical Systems Biology, Heidelberg, Germany., Hoffmann P; Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.; Department of Internal Medicine III, University Hospital Regensburg, Germany., Hogquist K; Center for Immunology, University of Minnesota, Minneapolis, MN, USA., Holland T; Institute of Experimental Immunology, University of Bonn, Germany., Höllt T; Leiden Computational Biology Center, Leiden University Medical Center, Leiden, The Netherlands.; Computer Graphics and Visualization, Department of Intelligent Systems, TU Delft, Delft, The Netherlands., Holmdahl R; Karolinska Institutet Biomedicum, Solna, Sweden., Hombrink P; Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands., Houston JP; Department of Chemical & Materials Engineering, New Mexico State University, Las Cruces, NM, USA., Hoyer BF; Rheumatologie/Klinische Immunologie, Klinik für Innere Medizin I und Exzellenzzentrum Entzündungsmedizin, Universitätsklinikum Schleswig-Holstein, Kiel, Germany., Huang B; Department of Immunology & National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China., Huang FP; Institute for Advanced Study (IAS), Shenzhen University, Shenzhen, China., Huber JE; Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany., Huehn J; Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Hundemer M; Department of Hematology, Oncology and Rheumatology, University Heidelberg, Heidelberg, Germany., Hunter CA; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA., Hwang WYK; Department of Hematology, Singapore General Hospital, Singapore.; Cancer & Stem Cell Biology, Duke-NUS Medical School, Singapore.; Executive Offices, National Cancer Centre Singapore, Singapore., Iannone A; Department of Diagnostic Medicine, Clinical and Public Health, Univ. of Modena and Reggio Emilia, Modena, Italy., Ingelfinger F; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland., Ivison SM; Department of Surgery, The University of British Columbia, Vancouver, Canada.; BC Children's Hospital Research Institute, Vancouver, Canada., Jäck HM; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Jani PK; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Max Planck Institute for Infection Biology, Berlin, Germany., Jávega B; Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain., Jonjic S; Department of Histology and Embryology/Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia., Kaiser T; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Kalina T; Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic., Kamradt T; Jena University Hospital, Institute of Immunology, Jena, Germany., Kaufmann SHE; Max Planck Institute for Infection Biology, Berlin, Germany., Keller B; Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Center for Chronic Immunodeficiency, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany., Ketelaars SLC; Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands., Khalilnezhad A; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Khan S; Department of Immunology, University of Toronto, Toronto, ON, Canada., Kisielow J; Institute of Molecular Health Sciences, ETH Zurich, Zürich, Switzerland., Klenerman P; Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Knopf J; Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen., Koay HF; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., Kobow K; Department of Neuropathology, Universitätsklinikum Erlangen, Germany., Kolls JK; John W Deming Endowed Chair in Internal Medicine, Center for Translational Research in Infection and Inflammation Tulane School of Medicine, New Orleans, LA, USA., Kong WT; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore., Kopf M; Institute of Molecular Health Sciences, ETH Zurich, Zürich, Switzerland., Korn T; Department of Neurology, Technical University of Munich, Munich, Germany., Kriegsmann K; Department of Hematology, Oncology and Rheumatology, University Heidelberg, Heidelberg, Germany., Kristyanto H; Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands., Kroneis T; Division of Cell Biology, Histology & Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria., Krueger A; Institute for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany., Kühne J; Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany., Kukat C; FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany., Kunkel D; Flow & Mass Cytometry Core Facility, Charité - Universitätsmedizin Berlin and Berlin Institute of Health, Berlin, Germany.; BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin., Kunze-Schumacher H; Institute for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany., Kurosaki T; WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan., Kurts C; Institute of Experimental Immunology, University of Bonn, Germany., Kvistborg P; Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands., Kwok I; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.; School of Biological Sciences, Nanyang Technological University, Singapore., Landry J; Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Lantz O; INSERM U932, PSL University, Institut Curie, Paris, France., Lanuti P; Department of Medicine and Aging Sciences, Centre on Aging Sciences and Translational Medicine (Ce.S.I.-Me.T.), University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., LaRosa F; IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.; Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy., Lehuen A; Institut Cochin, CNRS8104, INSERM1016, Department of Endocrinology, Metabolism and Diabetes, Université de Paris, Paris, France., LeibundGut-Landmann S; Section of Immunology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland., Leipold MD; The Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, CA, USA., Leung LYT; Department of Immunology, University of Toronto, Toronto, ON, Canada., Levings MK; Department of Surgery, The University of British Columbia, Vancouver, Canada.; BC Children's Hospital Research Institute, Vancouver, Canada.; School of Biomedical Engineering, The University of British Columbia, Vancouver, Canada., Lino AC; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Dept. Medicine/Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Germany., Liotta F; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Litwin V; Caprion Biosciences, ImmuneCarta, Montréal, Quebec, Canada., Liu Y; Department of Immunology, University of Toronto, Toronto, ON, Canada., Ljunggren HG; Center for Infectious Medicine, Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, Stockholm, Sweden., Lohoff M; Inst. f. Med. Mikrobiology and Hospital Hygiene, University of Marburg, Germany., Lombardi G; King's College London, 'Peter Gorer' Department of Immunobiology, London, UK., Lopez L; Beckman Coulter, Inc, Miami, FL, USA., López-Botet M; IMIM(Hospital de Mar Medical Research Institute), University Pompeu Fabra, Barcelona, Spain., Lovett-Racke AE; Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USA., Lubberts E; Department of Rheumatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands., Luche H; Centre d'Immunophénomique - CIPHE (PHENOMIN), Aix Marseille Université (UMS3367), Inserm (US012), CNRS (UMS3367), Marseille, France., Ludewig B; Institute of Immunobiology, Kantonsspital St.Gallen, St. Gallen, Switzerland., Lugli E; Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy.; Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy., Lunemann S; Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany., Maecker HT; Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA., Maggi L; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Maguire O; Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA., Mair F; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA, USA., Mair KH; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.; Christian Doppler Laboratory for Optimized Prediction of Vaccination Success in Pigs, Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria., Mantovani A; Istituto Clinico Humanitas IRCCS and Humanitas University, Pieve Emanuele, Milan, Italy.; William Harvey Research Institute, Queen Mary University, London, United Kingdom., Manz RA; Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany., Marshall AJ; Department of Immunology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada., Martínez-Romero A; Cytomics Technological Service, Principe Felipe Research Center, Valencia, Spain., Martrus G; Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany., Marventano I; IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.; Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy., Maslinski W; National Institute of Geriatrics, Rheumatology and Rehabilitation, Department of Pathophysiology and Immunology, Warsaw, Poland., Matarese G; Treg Cell Lab, Dipartimento di Medicina Molecolare e Biotecologie Mediche, Università di Napoli Federico II and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), Napoli, Italy., Mattioli AV; Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.; Lab of Clinical and Experimental Immunology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy., Maueröder C; Cell Clearance in Health and Disease Lab, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Mazzoni A; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., McCluskey J; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia., McGrath M; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., McGuire HM; Ramaciotti Facility for Human Systems Biology, and Discipline of Pathology, The University of Sydney, Camperdown, Australia., McInnes IB; Institute of Infection Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, UK., Mei HE; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Melchers F; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Max Planck Institute for Infection Biology, Berlin, Germany., Melzer S; Clinical Trial Center Leipzig, University Leipzig, Leipzig, Germany., Mielenz D; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Miller SD; Interdepartmental Immunobiology Center, Dept. of Microbiology-Immunology, Northwestern Univ. Medical School, Chicago, IL, USA., Mills KHG; Trinity College Dublin, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland., Minderman H; Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA., Mjösberg J; Center for Infectious Medicine, Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, Stockholm, Sweden.; Department of Clinical and Experimental Medine, Linköping University, Linköping, Sweden., Moore J; Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA., Moran B; Trinity College Dublin, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland., Moretta L; Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy., Mosmann TR; David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA., Müller S; Centre for Environmental Research - UFZ, Department Environmental Microbiology, Leipzig, Germany., Multhoff G; Institute for Innovative Radiotherapy (iRT), Experimental Immune Biology, Helmholtz Zentrum München, Neuherberg, Germany.; Radiation Immuno-Oncology Group, Center for Translational Cancer Research Technische Universität München (TranslaTUM), Klinikum rechts der Isar, Munich, Germany., Muñoz LE; Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen., Münz C; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.; Comprehensive Cancer Center Zurich, Switzerland., Nakayama T; Department of Immunology, Graduate School of Medicine, Chiba University, Chiba city, Chiba, Japan., Nasi M; Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy., Neumann K; Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Ng LG; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; School of Biological Sciences, Nanyang Technological University, Singapore.; Discipline of Dermatology, University of Sydney, Sydney, New South Wales, Australia.; State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China., Niedobitek A; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Nourshargh S; Barts and The London School of Medicine and Dentistry, Queen Mary University of London, UK., Núñez G; Department of Pathology and Rogel Cancer Center, the University of Michigan, Ann Arbor, Michigan, USA., O'Connor JE; Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain., Ochel A; Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Oja A; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands., Ordonez D; Flow Cytometry Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Orfao A; Department of Medicine, Cancer Research Centre (IBMCC-CSIC/USAL), Cytometry Service, University of Salamanca, CIBERONC and Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain., Orlowski-Oliver E; Burnet Institute, AMREP Flow Cytometry Core Facility, Melbourne, Victoria, Australia., Ouyang W; Inflammation and Oncology, Research, Amgen Inc, South San Francisco, USA., Oxenius A; ETH Zurich, Institute of Microbiology, Zurich, Switzerland., Palankar R; Department of Transfusion Medicine, Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany., Panse I; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Pattanapanyasat K; Center of Excellence for Flow Cytometry, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand., Paulsen M; Flow Cytometry Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Pavlinic D; Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Penter L; Department of Hematology, Oncology, and Tumor Immunology, Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany., Peterson P; Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia., Peth C; Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Petriz J; Functional Cytomics Group, Josep Carreras Leukaemia Research Institute, Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB, Badalona, Spain., Piancone F; IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.; Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy., Pickl WF; Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Piconese S; Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy.; Istituto Pasteur - Fondazione Cenci Bolognetti, Rome, Italy., Pinti M; Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy., Pockley AG; John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK.; Chromocyte Limited, Electric Works, Sheffield, UK., Podolska MJ; Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.; Department for Internal Medicine 3, Institute for Rheumatology and Immunology, AG Munoz, Universitätsklinikum Erlangen, Erlangen, Germany., Poon Z; Department of Hematology, Singapore General Hospital, Singapore., Pracht K; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Prinz I; Institute of Immunology, Hannover Medical School, Hannover, Germany., Pucillo CEM; Department of Immunology, University of Udine, Udine, Italy., Quataert SA; David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA., Quatrini L; Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy., Quinn KM; School of Biomedical and Health Sciences, RMIT University, Bundoora, Victoria, Australia.; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia., Radbruch H; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany., Radstake TRDJ; Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., Rahmig S; Regeneration in Hematopoiesis, Leibniz-Institute on Aging, Fritz-Lipmann-Institute (FLI), Jena, Germany., Rahn HP; Preparative Flow Cytometry, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany., Rajwa B; Bindley Biosciences Center, Purdue University, West Lafayette, IN, USA., Ravichandran G; Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Raz Y; Department of Internal Medicine, Groene Hart Hospital, Gouda, The Netherlands., Rebhahn JA; David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA., Recktenwald D; Desatoya LLC, Reno, NV, USA., Reimer D; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Reis e Sousa C; Immunobiology Laboratory, The Francis Crick Institute, London, UK., Remmerswaal EBM; Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.; Renal Transplant Unit, Division of Internal Medicine, Academic Medical Centre, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands., Richter L; Core Facility Flow Cytometry, Biomedical Center, Ludwig-Maximilians-University Munich, Germany., Rico LG; Functional Cytomics Group, Josep Carreras Leukaemia Research Institute, Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB, Badalona, Spain., Riddell A; Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, UK., Rieger AM; Department of Medical Microbiology and Immunology, University of Alberta, Alberta, Canada., Robinson JP; Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN, USA., Romagnani C; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Charité - Universitätsmedizin Berlin, Medical Department I, Division of Gastroenterology, Infectiology and Rheumatology, Berlin, Germany., Rubartelli A; Cell Biology Unit, IRCCS Ospedale Policlinico San Martino, Genova, Italy., Ruland J; Institut für Klinische Chemie und Pathobiochemie, Fakultät für Medizin, Technische Universität München, München, Germany., Saalmüller A; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria., Saeys Y; Data Mining and Modeling for Biomedicine, VIB-UGent Center for Inflammation Research, Ghent, Belgium.; Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium., Saito T; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan., Sakaguchi S; WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan., Sala-de-Oyanguren F; Flow Cytometry Facility, Ludwig Cancer Institute, Faculty of Medicine and Biology, University of Lausanne, Epalinges, Switzerland., Samstag Y; Heidelberg University, Institute of Immunology, Section of Molecular Immunology, Heidelberg, Germany., Sanderson S; Translational Immunology Laboratory, NIHR BRC, University of Oxford, Kennedy Institute of Rheumatology, Oxford, UK., Sandrock I; Institute of Immunology, Hannover Medical School, Hannover, Germany., Santoni A; Department of Molecular Medicine, Sapienza University of Rome, IRCCS, Neuromed, Pozzilli, Italy., Sanz RB; Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany., Saresella M; IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.; Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy., Sautes-Fridman C; Centre de Recherches des Cordeliers, Equipe Cancer et Immunité anti-tumorale, Paris, France., Sawitzki B; Charité - Universitätsmedizin Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Germany., Schadt L; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.; Comprehensive Cancer Center Zurich, Switzerland., Scheffold A; Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany., Scherer HU; Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands., Schiemann M; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany., Schildberg FA; Clinic for Orthopedics and Trauma Surgery, University Hospital Bonn, Bonn, Germany., Schimisky E; R&D Reagents, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Schlitzer A; Quantitative Systems Biology, Life & Medical Sciences Institute, University of Bonn, Bonn, Germany., Schlosser J; Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany., Schmid S; Internal Medicine I, University Hospital Regensburg, Germany., Schmitt S; Flow Cytometry Core Facility, German Cancer Research Centre (DKFZ), Heidelberg, Germany., Schober K; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany., Schraivogel D; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Schuh W; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Schüler T; Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany., Schulte R; University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK., Schulz AR; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany., Schulz SR; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Scottá C; King's College London, 'Peter Gorer' Department of Immunobiology, London, UK., Scott-Algara D; Institut Pasteur, Cellular Lymphocytes Biology, Immunology Departement, Paris, France., Sester DP; TRI Flow Cytometry Suite (TRI.fcs), Translational Research Institute, Wooloongabba, QLD, Australia., Shankey TV; AsedaSciences, West Lafayette, IN, USA., Silva-Santos B; Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Portugal., Simon AK; Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK., Sitnik KM; Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Sozzani S; Dept. Molecular Translational Medicine, University of Brescia, Brescia, Italy., Speiser DE; Department of Oncology, University of Lausanne and CHUV, Epalinges, Switzerland., Spidlen J; BD Life Sciences, Ashland, OR, USA., Stahlberg A; Lundberg Laboratory for Cancer, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden., Stall AM; BD Life Sciences, San Diego, CA, USA., Stanley N; Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences; and Pediatrics, Stanford University, Stanford, CA, USA., Stark R; Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands., Stehle C; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Charité - Universitätsmedizin Berlin, Medical Department I, Division of Gastroenterology, Infectiology and Rheumatology, Berlin, Germany., Steinmetz T; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., Stockinger H; Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Takahama Y; National Institutes of Health, Bethesda, MD, USA., Takeda K; WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan., Tan L; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Tárnok A; Departement for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.; Institute for Medical Informatics, Statistics and Epidemiology (IMISE), University of Leipzig, Leipzig, Germany.; Department of Precision Instruments, Tsinghua University, Beijing, China., Tiegs G; Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Toldi G; MDQuest Ltd., Szeged, Hungary., Tornack J; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; BioGenes GmbH, Berlin, Germany., Traggiai E; Novartis Biologics Center, Mechanistic Immunology Unit, Novartis Institute for Biomedical Research, NIBR, Basel, Switzerland., Trebak M; Department of Cellular and Molecular Physiology, Penn State University College of Medicine, PA, United States., Tree TIM; Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.; National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health Service, Foundation Trust and King's College London, UK., Trotter J; BD Life Sciences, San Diego, CA, USA., Trowsdale J; Department of Pathology, University of Cambridge, Cambridge, UK., Tsoumakidou M; Institute of Bioinnovation, BSRC Alexander Fleming, Vari, Greece., Ulrich H; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP, Brazil., Urbanczyk S; Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany., van de Veen W; Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.; Christine Kühne Center for Allergy Research and Education (CK-CARE), Davos, Switzerland., van den Broek M; Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.; Comprehensive Cancer Center Zurich, Switzerland., van der Pol E; Vesicle Observation Center, Biomedical Engineering & Physics, Laboratory Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, The Netherlands., Van Gassen S; Data Mining and Modeling for Biomedicine, VIB-UGent Center for Inflammation Research, Ghent, Belgium.; Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium., Van Isterdael G; VIB Flow Core, VIB Center for Inflammation Research, Ghent, Belgium., van Lier RAW; Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands., Veldhoen M; Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Portugal., Vento-Asturias S; Institute of Experimental Immunology, University of Bonn, Germany., Vieira P; Unit Lymphopoiesis, Department of Immunology, Institut Pasteur, Paris, France., Voehringer D; Department of Infection Biology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Erlangen, Germany., Volk HD; BIH Center for Regenerative Therapies (BCRT) Charité Universitätsmedizin Berlin and Berlin Institute of Health, Core Unit ImmunoCheck., von Borstel A; Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, Victoria, Australia., von Volkmann K; A·P·E-Applied Physics & Electronics, Berlin, Germany., Waisman A; Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany., Walker RV; Flow Cytometry Facility, Babraham Institute, Cambridge, UK., Wallace PK; Roswell Park Comprehensive Cancer Center, Elm and Carlton Streets, Buffalo, NY, USA., Wang SA; Dept of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA., Wang XM; The Scientific Platforms, the Westmead Institute for Medical Research, the Westmead Research Hub, Westmead, New South Wales, Australia., Ward MD; Thermo Fisher Scientific, Eugene, Oregon, USA., Ward-Hartstonge KA; Department of Surgery, The University of British Columbia, Vancouver, Canada., Warnatz K; Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Center for Chronic Immunodeficiency, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany., Warnes G; Flow Cytometry Core Facility, Blizard Institute, Queen Mary London University, London, UK., Warth S; BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin., Waskow C; Regeneration in Hematopoiesis, Leibniz-Institute on Aging, Fritz-Lipmann-Institute (FLI), Jena, Germany.; Faculty of Biological Sciences, Friedrich Schiller University Jena, Jena, Germany., Watson JV; Medinfomatics Ltd., West Drayton, Middlesex, UK., Watzl C; Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo), Dortmund, Germany., Wegener L; Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Weisenburger T; Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany., Wiedemann A; Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.; Dept. Medicine/Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Germany., Wienands J; Institute for Cellular & Molecular Immunology, University Medical Center Göttingen, Göttingen, Germany., Wilharm A; Institute of Immunology, Hannover Medical School, Hannover, Germany., Wilkinson RJ; Department of Infectious Disease, Imperial College London, UK.; Wellcome Centre for Infectious Diseases Research in Africa and Department of Medicine, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Republic of South Africa.; Tuberculosis Laboratory, The Francis Crick Institute, London, UK., Willimsky G; Cooperation Unit for Experimental and Translational Cancer Immunology, Institute of Immunology (Charité - Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ), Berlin, Germany., Wing JB; WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan., Winkelmann R; Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany., Winkler TH; Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany., Wirz OF; Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland., Wong A; Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore., Wurst P; University Bonn, Medical Faculty, Bonn, Germany., Yang JHM; Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.; National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health Service, Foundation Trust and King's College London, UK., Yang J; Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany., Yazdanbakhsh M; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands., Yu L; BD Biosciences, San Jose, CA, USA., Yue A; School of Computing Science, Simon Fraser University, Burnaby, Canada., Zhang H; Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK., Zhao Y; Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan, China., Ziegler SM; Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany., Zielinski C; German Center for Infection Research (DZIF), Munich, Germany.; Institute of Virology, Technical University of Munich, Munich, Germany.; TranslaTUM, Technical University of Munich, Munich, Germany., Zimmermann J; Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland., Zychlinsky A; Max Planck Institute for Infection Biology, Berlin, Germany.
Jazyk:	angličtina
Zdroj:	European journal of immunology [Eur J Immunol] 2019 Oct; Vol. 49 (10), pp. 1457-1973.
DOI:	10.1002/eji.201970107
Abstrakt:	These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. (© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze:	MEDLINE
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