High-fat diet-induced dysregulation of ovarian gene expression is restored with chronic omega-3 fatty acid supplementation.

Autor: Hohos NM; Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO, USA., Elliott EM; Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO, USA., Cho KJ; Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO, USA., Lin IS; Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO, USA., Rudolph MC; Department of Medicine, Division of Endocrinology, Metabolism and Diabetes, University of Colorado School of Medicine, 12700 East 19th Ave, Aurora, CO, 80045, USA., Skaznik-Wikiel ME; Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Aurora, CO, USA. Electronic address: malgorzata.skaznik-wikiel@cuanschutz.edu.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2020 Jan 01; Vol. 499, pp. 110615. Date of Electronic Publication: 2019 Oct 16.
DOI: 10.1016/j.mce.2019.110615
Abstrakt: Chronic high-fat diet (HFD) consumption causes ovarian dysfunction in rodents. Acute dietary treatment with docosahexaenoic acid (DHA) increases oocyte quality and ovarian reserve at advanced reproductive age. We hypothesized that DHA supplementation after HFD exposure reverses HFD-induced ovarian defects. We conducted a dietary intervention with reversal to chow, DHA-supplemented chow, or DHA-supplemented HFD after HFD consumption. After 10 weeks, HFD-fed mice had impaired estrous cyclicity, decreased primordial follicles, and altered ovarian expression of 24 genes compared to chow controls. Diet reversal to either chow or chow + DHA restored estrous cyclicity, however only chow + DHA appeared to mitigated the impact of HFD on ovarian reserve. All dietary interventions restored HFD-dysregulated gene expression to chow levels. We found no association between follicular fluid DHA levels and ovarian reserve. In conclusion our data suggest some benefit of DHA supplementation after HFD, particularly in regards to ovarian gene expression, however complete restoration of ovarian function was not achieved.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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