Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa.

Autor:	Cantero-Pérez J; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Grau-Expósito J; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Serra-Peinado C; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Rosero DA; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Luque-Ballesteros L; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Astorga-Gamaza A; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Castellví J; Pathology Department, Hospital Universitari Vall d'Hebron, UAB, Barcelona, Spain., Sanhueza T; Pathology Department, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain., Tapia G; Pathology Department, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain., Lloveras B; Pathology Department, Hospital del Mar, Parc de Salut Mar, Universitat Autònoma de Barcelona, Barcelona, Spain., Fernández MA; Flow Cytometry Facility, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Spain., Prado JG; AIDS Research Institute IrsiCaixa, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain., Solé-Sedeno JM; Obstetrics and Gynecology Department, Hospital del Mar, Parc de Salut Mar, Universitat Autònoma de Barcelona, Barcelona, Spain., Tarrats A; Department of Obstetrics and Gynecology, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain., Lecumberri C; Department of Obstetrics and Gynecology, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain., Mañalich-Barrachina L; Department of Obstetrics and Gynecology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain., Centeno-Mediavilla C; Department of Obstetrics and Gynecology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain., Falcó V; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Buzon MJ; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain. mariajose.buzon@vhir.org., Genescà M; Infectious Diseases Department, Hospital Universitari Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain. meritxell.genesca@vhir.org.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2019 Oct 18; Vol. 10 (1), pp. 4739. Date of Electronic Publication: 2019 Oct 18.
DOI:	10.1038/s41467-019-12732-2
Abstrakt:	HIV viral reservoirs are established very early during infection. Resident memory T cells (T RM ) are present in tissues such as the lower female genital tract, but the contribution of this subset of cells to the pathogenesis and persistence of HIV remains unclear. Here, we show that cervical CD4 + T RM display a unique repertoire of clusters of differentiation, with enrichment of several molecules associated with HIV infection susceptibility, longevity and self-renewing capacities. These protein profiles are enriched in a fraction of CD4 + T RM expressing CD32. Cervical explant models show that CD4 + T RM preferentially support HIV infection and harbor more viral DNA and protein than non-T RM . Importantly, cervical tissue from ART-suppressed HIV + women contain high levels of viral DNA and RNA, being the T RM fraction the principal contributor. These results recognize the lower female genital tract as an HIV sanctuary and identify CD4 + T RM as primary targets of HIV infection and viral persistence. Thus, strategies towards an HIV cure will need to consider T RM phenotypes, which are widely distributed in tissues.
Databáze:	MEDLINE
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