Living myofibroblast-silicon composites for probing electrical coupling in cardiac systems.

Autor:	Rotenberg MY; The James Franck Institute, The University of Chicago, Chicago, IL 60637; hrotenberg@uchicago.edu btian@uchicago.edu., Yamamoto N; Department of Chemistry, The University of Chicago, Chicago, IL 60637., Schaumann EN; Department of Chemistry, The University of Chicago, Chicago, IL 60637., Matino L; Tissue Electronics, Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, 80125 Naples, Italy.; Department of Chemical Materials and Industrial Production Engineering, University of Naples Federico II, 80125 Naples, Italy., Santoro F; Tissue Electronics, Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, 80125 Naples, Italy., Tian B; The James Franck Institute, The University of Chicago, Chicago, IL 60637; hrotenberg@uchicago.edu btian@uchicago.edu.; Department of Chemistry, The University of Chicago, Chicago, IL 60637.; The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Nov 05; Vol. 116 (45), pp. 22531-22539. Date of Electronic Publication: 2019 Oct 17.
DOI:	10.1073/pnas.1913651116
Abstrakt:	Traditional bioelectronics, primarily comprised of nonliving synthetic materials, lack cellular behaviors such as adaptability and motility. This shortcoming results in mechanically invasive devices and nonnatural signal transduction across cells and tissues. Moreover, resolving heterocellular electrical communication in vivo is extremely limited due to the invasiveness of traditional interconnected electrical probes. In this paper, we present a cell-silicon hybrid that integrates native cellular behavior (e.g., gap junction formation and biosignal processing) with nongenetically enabled photosensitivity. This hybrid configuration allows interconnect-free cellular modulation with subcellular spatial resolution for bioelectric studies. Specifically, we hybridize cardiac myofibroblasts with silicon nanowires and use these engineered hybrids to synchronize the electrical activity of cardiomyocytes, studying heterocellular bioelectric coupling in vitro. Thereafter, we inject the engineered myofibroblasts into heart tissues and show their ability to seamlessly integrate into contractile tissues in vivo. Finally, we apply local photostimulation with high cell specificity to tackle a long-standing debate regarding the existence of myofibroblast-cardiomyocyte electrical coupling in vivo. Competing Interests: The authors declare no competing interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu