Rheumatoid arthritis induces enteric neurodegeneration and jejunal inflammation, and quercetin promotes neuroprotective and anti-inflammatory actions.

Autor: Piovezana Bossolani GD; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Silva BT; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Colombo Martins Perles JV; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Lima MM; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Vieira Frez FC; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Garcia de Souza SR; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Sehaber-Sierakowski CC; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil., Bersani-Amado CA; Department of Pharmacology and Therapeutic, Universidade Estadual de Maringá, Máringá, Paraná, Brazil., Zanoni JN; Department of Morphological Sciences, Universidade Estadual de Maringá, 87020-900, Maringá, Paraná, Brazil. Electronic address: jnzanoni@uem.br.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2019 Dec 01; Vol. 238, pp. 116956. Date of Electronic Publication: 2019 Oct 14.
DOI: 10.1016/j.lfs.2019.116956
Abstrakt: Aims: The aim of our study was to study the pathological mechanisms induced by the rheumatoid arthritis (RA) on the Enteric Nervous System (ENS).
Main Methods: We evaluated the effect of the chronic arthritis and its treatment with 50 mg/kg quercetin alone (AQ) and combined with 17.5 mg/kg ibuprofen (AIQ) for 60 days on neurons, glial cells and intestinal wall. Other groups were used: control (C), arthritic (A) and arthritic treated with 17.5 mg/kg ibuprofen (AI). After 60 days, the jejunum was removed and processed for immunohistochemical techniques. Immunostainings were performed for HuC/D and S100 (myenteric and submucosal plexuses), and GFAP (only myenteric plexus), while immunolabeling for CD45 and CD20 lymphocytes was performed using cryosections. Western blot was performed for GDNF, S100 and GFAP.
Key Findings: A group yielded a remarkable density decrease of the neurons and glial cells with morphometric changes in the myenteric and submucosal plexuses, reduction of the GDNF expression and GFAP-related parameters (GFAP expression, occupancy area and GFAP-expressing glial cells) and intestinal inflammation and atrophy of the mucosa and intestinal wall. AQ group substantially reversed most of these effects, except for intestinal atrophy of the jejunum. The AI and AIQ groups displayed lower beneficial results than AQ for parameters related to the neurons and glial cells, although AIQ did not prevent the inflammation of the mucosa.
Significance: The severe chronic rheumatoid arthritis induced severe effects on ENS and mucosa, and quercetin treatment continues to be an important antioxidant supplement preventing the progression of the RA severity.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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