Genotyping by Sequencing of Cultivated Lentil ( Lens culinaris Medik.) Highlights Population Structure in the Mediterranean Gene Pool Associated With Geographic Patterns and Phenotypic Variables.

Autor: Pavan S; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy.; Institute of Biomedical Technologies, National Research Council (CNR), Bari, Italy., Bardaro N; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy., Fanelli V; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy., Marcotrigiano AR; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy., Mangini G; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy., Taranto F; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy.; CREA Research Centre for Cereal and Industrial Crops (CREA-CI), Foggia, Italy., Catalano D; Institute of Biomedical Technologies, National Research Council (CNR), Bari, Italy., Montemurro C; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy., De Giovanni C; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy., Lotti C; Department of Agricultural, Food and Environmental Sciences, University of Foggia, Foggia, Italy., Ricciardi L; Department of Soil, Plant and Food Science, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Bari, Italy.
Jazyk: angličtina
Zdroj: Frontiers in genetics [Front Genet] 2019 Sep 18; Vol. 10, pp. 872. Date of Electronic Publication: 2019 Sep 18 (Print Publication: 2019).
DOI: 10.3389/fgene.2019.00872
Abstrakt: Cultivated lentil ( Lens culinaris Medik.) is one of the oldest domesticated crops and one of the most important grain legumes worldwide. The Mediterranean Basin holds large part of lentil biodiversity; however, no genetic structure was defined within the Mediterranean gene pool. In this study, we used high-throughput genotyping by sequencing to resolve the genetic structure of the Mediterranean ex situ lentil collection held at the Italian National Research Council. Sequencing of a 188-plex genotyping-by-sequencing library and bioinformatics treatment of data yielded 6,693 single nucleotide polymorphisms. Analysis of nonredundant genotypes with nonparametric and parametric methods highlighted the occurrence of five highly differentiated genetic clusters. Clustering could be related to geographic patterns and phenotypic traits, indicating that post-domestication routes introducing cultivation in Mediterranean countries and selection were major forces shaping lentil population structure. The estimation of the fixation index F ST at individual single nucleotide polymorphism loci allowed the identification of distinctive alleles across clusters, suggesting the possibility to set up molecular keys for the assignment of lentil germplasm to specific genetic groups. Finally, significant associations between markers and phenotypic data were identified. Overall, the results of this study are of major importance for lentil conservation genetics and breeding and provide insights on the lentil evolutionary history.
(Copyright © 2019 Pavan, Bardaro, Fanelli, Marcotrigiano, Mangini, Taranto, Catalano, Montemurro, De Giovanni, Lotti and Ricciardi.)
Databáze: MEDLINE
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