How Boundaries Form: Linked Nonautonomous Feedback Loops Regulate Pattern Formation in Yeast Colonies.

Autor:	Piccirillo S; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110., McCune AH; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110., Dedert SR; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110., Kempf CG; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110., Jimenez B; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110., Solst SR; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110., Tiede-Lewis LM; UMKC Department of Oral and Craniofacial Sciences, University of Missouri-Kansas City, Missouri 64108., Honigberg SM; Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110 honigbergs@umkc.edu.
Jazyk:	angličtina
Zdroj:	Genetics [Genetics] 2019 Dec; Vol. 213 (4), pp. 1373-1386. Date of Electronic Publication: 2019 Oct 16.
DOI:	10.1534/genetics.119.302700
Abstrakt:	Under conditions in which budding yeast form colonies and then undergo meiosis/sporulation, the resulting colonies are organized such that a sharply defined layer of meiotic cells overlays a layer of unsporulated cells termed "feeder cells." This differentiation pattern requires activation of both the Rlm1/cell-wall integrity pathway and the Rim101/alkaline-response pathway. In the current study, we analyzed the connection between these two signaling pathways in regulating colony development by determining expression patterns and cell-autonomy relationships. We present evidence that two parallel cell-nonautonomous positive-feedback loops are active in colony patterning, an Rlm1-Slt2 loop active in feeder cells and an Rim101-Ime1 loop active in meiotic cells. The Rlm1-Slt2 loop is expressed first and subsequently activates the Rim101-Ime1 loop through a cell-nonautonomous mechanism. Once activated, each feedback loop activates the cell fate specific to its colony region. At the same time, cell-autonomous mechanisms inhibit ectopic fates within these regions. In addition, once the second loop is active, it represses the first loop through a cell-nonautonomous mechanism. Linked cell-nonautonomous positive-feedback loops, by amplifying small differences in microenvironments, may be a general mechanism for pattern formation in yeast and other organisms. (Copyright © 2019 by the Genetics Society of America.)
Databáze:	MEDLINE
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