| Autor: |
Pastva O; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Chrastinová L; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Bocková M; Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic , Chaberská 57 , 18251 Prague , Czech Republic., Kotlín R; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Suttnar J; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Hlaváčková A; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Štikarová J; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Ceznerová E; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Čermák J; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic., Homola J; Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic , Chaberská 57 , 18251 Prague , Czech Republic., Dyr JE; Institute of Hematology and Blood Transfusion , U Nemocnice 1 , 12820 Prague , Czech Republic. |
| Abstrakt: |
The onset and progression of numerous serious diseases (e.g., various types of malignancies, neurodegenerative diseases, and cardiac diseases) are, on a molecular level, associated with protein modifications and misfolding. Current methods for the detection of misfolded proteins are not able to detect the whole misfolded subproteome and, moreover, are rather laborious and time consuming. Herein, we report on a novel simple method for the detection of misfolded proteins employing a surface plasmon resonance (SPR) biosensor and heat shock protein 70 (Hsp70) that recognizes and traps misfolded proteins in a nucleotide-dependent manner. We use this method for the detection of misfolded proteins in blood plasma of patients with various subtypes of myelodysplastic syndromes (MDS) and healthy donors. Our results reveal significantly elevated levels of misfolded proteins in the two stages of MDS that are most affected by oxidative stress: low-risk (RARS) and intermediate-risk (RCMD) patients. This approach can be extended to a variety of diseases and provides unique insights into the thus far unexplored area of blood proteome. |
