Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.

Autor: Seyffert M; Institute of Virology, University of Zurich, Zürich, Switzerland. michael.seyffert@uzh.ch., Fraefel C; Institute of Virology, University of Zurich, Zürich, Switzerland.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2060, pp. 365-376.
DOI: 10.1007/978-1-4939-9814-2_22
Abstrakt: The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows for monitoring virus replication in a spatial and temporal fashion. Here, we describe the multifluorescent live analysis of the multicompartment Herpes Simplex Virus Type-1 (HSV-1) by live cell confocal laser scanning microscopy.
Databáze: MEDLINE