Expression of protocadherin-γC4 protein in the rat brain.

Autor:	Miralles CP; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., Taylor MJ; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., Bear J Jr; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., Fekete CD; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., George S; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., Li Y; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., Bonhomme B; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., Chiou TT; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA., De Blas AL; Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA.
Jazyk:	angličtina
Zdroj:	The Journal of comparative neurology [J Comp Neurol] 2020 Apr 01; Vol. 528 (5), pp. 840-864. Date of Electronic Publication: 2019 Nov 06.
DOI:	10.1002/cne.24783
Abstrakt:	It has been proposed that the combinatorial expression of γ-protocadherins (Pcdh-γs) and other clustered protocadherins (Pcdhs) provides a code of molecular identity and individuality to neurons, which plays a major role in the establishment of specific synaptic connectivity and formation of neuronal circuits. Particular attention has been directed to the Pcdh-γ family, for which experimental evidence derived from Pcdh-γ-deficient mice shows that they are involved in dendrite self-avoidance, synapse development, dendritic arborization, spine maturation, and prevention of apoptosis of some neurons. Moreover, a triple-mutant mouse deficient in the three C-type members of the Pcdh-γ family (Pcdh-γC3, Pcdh-γC4, and Pcdh-γC5) shows a phenotype similar to the mouse deficient in whole Pcdh-γ family, indicating that the latter is largely due to the absence of C-type Pcdh-γs. The role of each individual C-type Pcdh-γ is not known. We have developed a specific antibody to Pcdh-γC4 to reveal the expression of this protein in the rat brain. The results show that although Pcdh-γC4 is expressed at higher levels in the embryo and earlier postnatal weeks, it is also expressed in the adult rat brain. Pcdh-γC4 is expressed in both neurons and astrocytes. In the adult brain, the regional distribution of Pcdh-γC4 immunoreactivity is similar to that of Pcdh-γC4 mRNA, being highest in the olfactory bulb, dentate gyrus, and cerebellum. Pcdh-γC4 forms puncta that are frequently apposed to glutamatergic and GABAergic synapses. They are also frequently associated with neuron-astrocyte contacts. The results provide new insights into the cell recognition function of Pcdh-γC4 in neurons and astrocytes. (© 2019 Wiley Periodicals, Inc.)
Databáze:	MEDLINE
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