Neurotoxicity mediated by oxidative stress caused by titanium dioxide nanoparticles in human neuroblastoma (SH-SY5Y) cells.

Autor: Ferraro SA; Center of Studies in Health and Environment, School of Science and Technology, National University of San Martín, San Martín, Buenos Aires, Argentina; National Council of Scientific and Technical Research (CONICET), Buenos Aires, Argentina. Electronic address: sebaferraro@gmail.com., Domingo MG; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Anatomía Patológica, Buenos Aires, Argentina; Research Fellow of the University of Buenos Aires, Buenos Aires, Argentina. Electronic address: marieladomingo@hotmail.com., Etcheverrito A; Center of Studies in Health and Environment, School of Science and Technology, National University of San Martín, San Martín, Buenos Aires, Argentina. Electronic address: abetcheverrito@gmail.com., Olmedo DG; National Council of Scientific and Technical Research (CONICET), Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Anatomía Patológica, Buenos Aires, Argentina. Electronic address: olmedodg@gmail.com., Tasat DR; Center of Studies in Health and Environment, School of Science and Technology, National University of San Martín, San Martín, Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Histología y Embriología, Buenos Aires, Argentina. Electronic address: dtasat@gmail.com.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2020 Jan; Vol. 57, pp. 126413. Date of Electronic Publication: 2019 Sep 25.
DOI: 10.1016/j.jtemb.2019.126413
Abstrakt: Background: Titanium is widely used in biomedicine. Due to biotribocorrosion, titanium dioxide (TiO 2 ) nanoparticles (NPs) can be released from the titanium implant surface, enter the systemic circulation, and migrate to various organs and tissues including the brain. A previous study showed that 5 nm TiO 2 NPs reached the highest concentration in the brain. Even though TiO 2 NPs are believed to possess low toxicity, little is known about their neurotoxic effects. The aim of the study was to evaluate in vitro the effects of 5 nm TiO 2 NPs on a human neuroblastoma (SH-SY5Y) cell line.
Methods: Cell cultures were divided into non-exposed and exposed to TiO 2 NPs for 24 h. The following were evaluated: reactive oxygen species (ROS) generation, apoptosis, cellular antioxidant response, endoplasmic reticulum stress and autophagy.
Results: Exposure to TiO 2 NPs induced ROS generation in a dose dependent manner, with values reaching up to 10 fold those of controls (p < 0.001). Nrf2 nuclear localization and autophagy, also increased in a dose dependent manner. Apoptosis increased by 4- to 10-fold compared to the control group, depending on the dose employed.
Conclusions: Our results show that TiO 2 NPs cause ROS increase, induction of ER stress, Nrf2 cytoplasmic translocation to the nucleus and apoptosis. Thus, neuroblastoma cell response to TiO 2 NPs may be associated with an imbalance of the oxidative metabolism where endoplasmic reticulum-mediated signal pathway seems to be the main neurotoxic mechanism.
(Copyright © 2019 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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