Rapid Centrifugation in the Routine Hemostasis Laboratory.

Autor:	Wolfensberger N; Department of Medical Oncology and Hematology, University Hospital Zurich, Zurich, Switzerland., Georgiou G; Unilabs Lausanne, Lausanne, Switzerland., Giabbani E; Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland., Reusser M; Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland., Njue LM; Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland., Fiedler M; University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland., Leichtle AB; University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.; Insel Data Science Center, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland., Nagler M; Department of Hematology and Central Hematology Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.; University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Jazyk:	angličtina
Zdroj:	Thrombosis and haemostasis [Thromb Haemost] 2019 Dec; Vol. 119 (12), pp. 2025-2033. Date of Electronic Publication: 2019 Oct 10.
DOI:	10.1055/s-0039-1696712
Abstrakt:	Background:  The use of short and uniform centrifugation schemes contributes significantly to the successful automation of laboratory procedures. It is however unclear if this is applicable to the hemostasis laboratory. Objectives:  This article assesses the accuracy of measurements obtained with a rapid, high-speed centrifugation scheme in a large set of hemostasis tests, covering the full spectrum of values obtained in clinical practice, and using meaningful statistical measures. Methods:  Two citrated plasma samples were obtained from consecutive patients of a tertiary hospital with suspected abnormal hemostasis tests and processed with two centrifugation schemes in parallel: 1,500 ×  g for 10 minutes and 3,137 ×  g for 7 minutes. The following tests were conducted: prothrombin time ( n  = 125), international normalized ratio ( n  = 146), activated partial thromboplastin time ( n  = 119), thrombin time ( n  = 105), fibrinogen ( n  = 125), factor (F)II ( n  = 69), FV ( n  = 64), FVII ( n  = 64), FX ( n  = 67), FVIII ( n  = 55), FIX ( n  = 37), FXI ( n  = 35), and FXIII ( n  = 20), D-dimer ( n  = 34), antithrombin ( n  = 31), anti-Xa activity ( n  = 30), von Willebrand antigen ( n  = 25), and von Willebrand activity (VWF:GPIbM; n  = 27). Results:  A wide range of results were obtained in all tests. Spearman's rank correlation coefficient was at least 0.95 for all tests except FV, FIX, and FXI. The coverage probability π at a given deviation index κ of 15% was above 0.9 for all tests except FV, FVII, FX, FVIII, FIX, FXI, and VWF:GPIbM, suggesting a lack of agreement. Conclusion:  Our results suggest that high-speed centrifugation is applicable to the majority of routine hemostasis parameters. The coverage probability was more sensitive than Spearman's rank correlation to detect disagreement among centrifugation schemes. Competing Interests: M.N. reports research support from Roche diagnostics, Stago, Technoclone, Pentapharm, and Siemens healthcare. G.G. reports advisory board participation for Gilead, Celgene, Takeda, Novartis, Roche, Amgen, Health Research and Consulting, Orpha Suisse. All other authors state that they have no conflict of interest. (Georg Thieme Verlag KG Stuttgart · New York.)
Databáze:	MEDLINE
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