Nutrigenomic effect of conjugated linoleic acid on growth and meat quality indices of growing rabbit.

Autor: Abdelatty AM; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Mohamed SA; Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt., Moustafa MMA; Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt., Al-Mokaddem AK; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza,Egypt., Baker MR; Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Elolimy AA; Department of Animal Sciences, University of Illinois, Urbana, IL, United States of America., Elmedany SA; Regional Center for Food and Feed, Agriculture Research Center; Giza, Egypt., Hussein S; Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Farid OAA; Department of Physiology, National Organization for Drug Control and Research, Giza, Egypt., Sakr OG; Animal Production Department (Poultry Physiology), Faculty of Agriculture, Cairo University, Giza, Egypt., Elhady MA; Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Bionaz M; Department of Animal and Rangeland sciences, Oregon State University, Oregon, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Oct 10; Vol. 14 (10), pp. e0222404. Date of Electronic Publication: 2019 Oct 10 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0222404
Abstrakt: Conjugated linoleic acid was detected in rabbit caecotrophs, due to the presence of microbial lipid activity in rabbit cecum. However, the effect of CLA as a functional food in growing rabbit is not well established. Therefore, this study was conducted to determine the effect of CLA on production, meat quality, and its nutrigenomic effect on edible parts of rabbit carcass including skeletal muscle, liver, and adipose tissue. Therefore, seventy five weaned V-Line male rabbits, 30 days old, were randomly allocated into three dietary treatments receiving either basal control diet, diet supplemented with 0.5% (CLAL), or 1% CLA (CLAH). Total experimental period (63 d) was segmented into 7 days adaptation and 56 days experimental period. Dietary supplementation of CLA did not alter growth performance, however, the fat percentage of longissimus lumborum muscle was decreased, with an increase in protein and polyunsaturated fatty acids (PUFA) percentage. Saturated fatty acids (SFA) and mono unsaturated fatty acids (MUFA) were not increased in CLA treated groups. There was tissue specific sensing of CLA, since subcutaneous adipose tissue gene expression of PPARA was downregulated, however, CPT1A tended to be upregulated in liver of CLAL group only (P = 0.09). In skeletal muscle, FASN and PPARG were upregulated in CLAH group only (P ≤0.01). Marked cytoplasmic vacuolation was noticed in liver of CLAH group without altering hepatocyte structure. Adipocyte size was decreased in CLA fed groups, in a dose dependent manner (P <0.01). Cell proliferation determined by PCNA was lower (P <0.01) in adipose tissue of CLA groups. Our data indicate that dietary supplementation of CLA (c9,t11-CLA and t10,c12- CLA) at a dose of 0.5% in growing rabbit diet produce rabbit meat rich in PUFA and lower fat % without altering growth performance and hepatocyte structure.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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