Synthetic networks with tunable responsiveness, biodegradation, and molecular recognition for precision medicine applications.

Autor:	Clegg JR; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA., Irani AS; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA., Ander EW; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Ludolph CM; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Venkataraman AK; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA., Zhong JX; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Peppas NA; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.; Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, Austin, TX, USA.; Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA.; Department of Surgery and Perioperative Care, and Department of Pediatrics, Dell Medical School, The University of Texas at Austin, Austin, TX, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2019 Sep 27; Vol. 5 (9), pp. eaax7946. Date of Electronic Publication: 2019 Sep 27 (Print Publication: 2019).
DOI:	10.1126/sciadv.aax7946
Abstrakt:	Formulations and devices for precision medicine applications must be tunable and multiresponsive to treat heterogeneous patient populations in a calibrated and individual manner. We engineered modular poly(acrylamide-co-methacrylic acid) copolymers, cross-linked into multiresponsive nanogels with either a nondegradable or degradable disulfide cross-linker, that were customized via orthogonal chemistries to target biomarkers of an individual patient's disease or deliver multiple therapeutic modalities. Upon modification with functional small molecules, peptides, or proteins, these nanomaterials delivered methylene blue with environmental responsiveness, transduced visible light for photothermal therapy, acted as a functional enzyme, or promoted uptake by cells. In addition to quantifying the nanogels' composition, physicochemical characteristics, and cytotoxicity, we used a QCM-D method for characterizing nanomaterial degradation and a high-throughput assay for cellular uptake. In conclusion, we generated a tunable nanogel composition for precision medicine applications and new quantitative protocols for assessing the bioactivity of similar platforms. (Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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