Polymerase Chain Reaction and Its Application in the Diagnosis of Infectious Keratitis.

Autor:	Liu HY; Health Science Center, McGovern Medical School, University of Texas, Houston, TX, USA., Hopping GC; Health Science Center, McGovern Medical School, University of Texas, Houston, TX, USA., Vaidyanathan U; Health Science Center, McGovern Medical School, University of Texas, Houston, TX, USA., Ronquillo YC; John A. Moran Eye Center, Department of Ophthalmology and Visual Sciences, School of Medicine, University of Utah, Salt Lake City, UT, USA., Hoopes PC; John A. Moran Eye Center, Department of Ophthalmology and Visual Sciences, School of Medicine, University of Utah, Salt Lake City, UT, USA., Moshirfar M; John A. Moran Eye Center, Department of Ophthalmology and Visual Sciences, School of Medicine, University of Utah, Salt Lake City, UT, USA.; Utah Lions Eye Bank, Murray, UT, USA.; Hoopes Durrie Rivera Research Center, Hoopes Vision, Draper, UT, USA.
Jazyk:	angličtina
Zdroj:	Medical hypothesis, discovery & innovation ophthalmology journal [Med Hypothesis Discov Innov Ophthalmol] 2019 Fall; Vol. 8 (3), pp. 152-155.
Abstrakt:	PCR involves a repeating cycle of replication to amplify small segments of deoxyribonucleic acid (DNA). A novel application of this technique is microbial identification in infectious keratitis, one of the leading causes of blindness in the world. PCR is more sensitive than biological stains and culture, which are considered the current gold standards for diagnosing infectious keratitis. The diagnosis and treatment of infectious keratitis cost the United States millions of dollars in health expenditure. PCR may help offset that cost by allowing for individualized disease management and screening for multiple antibiotic-resistant genes. While beneficial, PCR demonstrates lower specificity rates compared to culture and stain, indicating its shortcomings; this can be overcome by performing PCR after narrowing the pool of potential microorganisms. This article examines the clinical utility of PCR in cases of infectious keratitis by evaluating its reliability, validity, associated costs, and indications. Competing Interests: Ethical issues have been completely observed by the authors. All named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship of this manuscript, take responsibility for the integrity of the work as a whole, and have given final approval for the version to be published. No conflict of interest has been presented. (© 2019, Author(s).)
Databáze:	MEDLINE
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