Consortia of bioactives in supercritical carbon dioxide extracts of mustard and small cardamom seeds lower serum cholesterol levels in rats: new leads for hypocholesterolaemic supplements from spices.

Autor: Chakraborty S; Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700032, India., Paul K; Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700032, India., Mallick P; Division of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, India., Pradhan S; Research Unit developed by Department of Physiology, Nutrition and Microbiology, Raja N.L. Khan Women's College, Midnapore 721102, West Bengal, India., Das K; Research Unit developed by Department of Physiology, Nutrition and Microbiology, Raja N.L. Khan Women's College, Midnapore 721102, West Bengal, India., Chakrabarti S; Division of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, India., Nandi DK; Research Unit developed by Department of Physiology, Nutrition and Microbiology, Raja N.L. Khan Women's College, Midnapore 721102, West Bengal, India., Bhattacharjee P; Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata 700032, India.
Jazyk: angličtina
Zdroj: Journal of nutritional science [J Nutr Sci] 2019 Sep 24; Vol. 8, pp. e32. Date of Electronic Publication: 2019 Sep 24 (Print Publication: 2019).
DOI: 10.1017/jns.2019.28
Abstrakt: Melatonin-rich and 1,8-cineole-rich extracts have been successfully obtained from yellow mustard (YM) and small cardamom (SC) seeds, respectively, employing green technology of supercritical CO 2 (SC-CO 2 ) extraction. Chemical profiling confirmed the presence of melatonin and 1,8-cineole and co-extractants in the respective extracts. Electron paramagnetic resonance spectroscopy attested strong antioxidant activities of the extracts foregoing pan-assay interference compounds involved in spectroscopic analysis. These extracts also exhibited synergistic efficacies greater than unity confirming antioxidant synergy among the co-extracted bioactives therein. To ascertain hypocholesterolaemic efficacies, these extracts were co-administered orally with Triton X (at the pre-optimised dose of 175 mg/kg body weight (BW)) to Wistar albino rats at doses of 550, 175 and 55 mg/kg BW. Serum total cholesterol levels in the rats were monitored on days 3, 7, 15 and 21. On day 21, total cholesterol level reduced appreciably by 49·44 % in rats treated with YM seed extract and by 48·95 % in rats treated with SC seed extract, comparable with atorvastatin-administered rats (51·09 %). Either extract demonstrated inhibitory effects on hepatic 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase activity. A molecular docking exercise identified specific compounds in the extracts which possessed binding affinities comparable with therapeutically used HMG-CoA reductase inhibitors. In silico and in vivo studies concertedly concluded that the consortium of bioactive components in the extracts cannot be considered as invalid metabolic panaceas and therefore these 'green' extracts could be safely subjected to clinical studies as preventive biotherapeutics for hypercholesterolaemia. These extracts could be consumed per se as hypocholesterolaemic supplements or could be ingredients of new spice-based therapeutic foods.
(© The Author(s) 2019.)
Databáze: MEDLINE
Externí odkaz: