Altered levels of circulating nuclear and mitochondrial DNA in patients with Papillary Thyroid Cancer.

Autor: Perdas E; Department of Cardiovascular Physiology, Faculty of Medicine, Medical University of Lodz, Lodz, Poland. ewelina.perdas@umed.lodz.pl., Stawski R; Department of Clinical Physiology, Faculty of Medicine, Medical University of Lodz, Lodz, Poland., Kaczka K; Department of General and Oncological Surgery, Medical University of Lodz, Lodz, Poland., Nowak D; Department of Clinical Physiology, Faculty of Medicine, Medical University of Lodz, Lodz, Poland., Zubrzycka M; Department of Cardiovascular Physiology, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Oct 08; Vol. 9 (1), pp. 14438. Date of Electronic Publication: 2019 Oct 08.
DOI: 10.1038/s41598-019-51000-7
Abstrakt: Papillary thyroid cancer is the most common thyroid cancer type. However, diagnostics based on fine needle biopsy cannot make a definitive diagnosis in 25% of thyroid nodules. Additionally, approximately 70% to 80% of thyroid lobectomies performed just for diagnostic purposes are benign. Despite this, biopsy still remains the main method of evaluation of thyroid nodules. Cell-free DNA (cf-DNA) measurement could give a new diagnostic opportunities which may reduce the number of unnecessary thyroid procedures. In this study, using a qPCR, we have examined the nuclear cf-DNA and mitochondrial cf-DNA in the plasma of 32 patients. We have found that the level of nuclear cf-DNA is almost 2-fold increased (median 3 089 vs. 1 872, p = 0.022), whereas mitochondrial cf-DNA content was significantly decreased in respect to healthy controls (median 44 992 vs. 92 220, p = 0.010). The ROC curve analysis showed high specificity for nuclear cf-DNA and mitochondrial cf-DNA, which may serve as a useful tool to decrease the number of unneeded surgeries. Our study reports the first epidemiological evidence for lower mitochondrial cf-DNA content in the patient group, what suggests that apart from nuclear cf-DNA also mitochondrial cf-DNA is affected by disease development.
Databáze: MEDLINE
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