Folate-chitosan nanoparticles triggered insulin cellular uptake and improved in vivo hypoglycemic activity.

Autor: El Leithy ES; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Cairo, Egypt; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Helwan University, Cairo, Egypt., Abdel-Bar HM; Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Menoufia, Egypt. Electronic address: hend.abdelbar@fop.usc.edu.eg., Ali RA; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2019 Nov 25; Vol. 571, pp. 118708. Date of Electronic Publication: 2019 Oct 05.
DOI: 10.1016/j.ijpharm.2019.118708
Abstrakt: The aim of this study was to prove a prolonged control of glucose levels, in rats, by the oral use of insulin folate-chitosan nanoparticles (FA-CS NPs). It was possible to prepare positively charged NPs with an average particle size of 288 ± 5.11 nm and >80% entrapment efficiency. The system was able to enhance the stability of insulin in presence of GIT enzymes, with less than 10% release at pH 1.2 and an 8 hr released amount of 38.92 ± 4.52% in PBS pH 7.4. A 5 fold enhancement in insulin intestinal permeability and cellular uptake over insulin solution was proven. The cellular uptake pathways was found to occur by several mechanisms. Besides, cell compatibility and absence of histopathological alterations was also demonstrated. Finally, a controlled blood glucose level for 8 h in rats. These results anticipated FA-CS NPs as a promising oral insulin candidate.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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