Disperse red 15 (DR15) impedes biofilm formation of uropathogenic Escherichia coli.

Autor: Miryala S; Biofilm Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India., Makala H; Molecular Motors Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India., Yadavali SP; Organic Synthesis Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India., Venkatasubramanian U; Molecular Motors Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India., Subbaiah N; Department of Chemistry, National Institute of Technology, Warangal, Telangana, India., Srinandan CS; Biofilm Biology Lab, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India; Centre for Research in Infectious Diseases, SASTRA Deemed University, Thanjavur, Tamil Nadu, India. Electronic address: srinandan@gmail.com.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2020 Jan; Vol. 138, pp. 103772. Date of Electronic Publication: 2019 Oct 04.
DOI: 10.1016/j.micpath.2019.103772
Abstrakt: Catheter associated urinary tract infection (CAUTI) is a highly prevalent hospital-acquired infection that is predominantly caused by uropathogenic Escherichia coli (UPEC). It adheres on catheter surface using type I pili as the initial step of pathogenesis that progresses to form biofilm. In this study, potential inhibitors against FimH adhesin of type I pili were screened computationally that yielded ten compounds. These were further validated in vitro against adhesion and biofilm formation. The compounds, 1-Amino-4-hydroxyanthraquinone (Disperse Red 15 or DR15) and 4-(4'-chloro-4-biphenylylsulfonylamino) benzoic acid (CB1) impaired adhesion and biofilm formation without inhibiting the planktonic growth. Also, both compounds inhibited cell assemblages like autoaggregation and swarming motility by unknown mechanisms. DR15 was further derivatised into N-(4-hydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl) undec-10-enamide that self-assembled with linseed oil, which was used as the coating material on urinary Foley catheters. The thin-film coating on the catheter did not leach when incubated in artificial urine and effectively restricted biofilm formation of UPEC. Altogether, the thin-film coating of urinary catheter with DR15 inhibited biofilm formation of UPEC and this application could potentially help to reduce CAUTI incidents in healthcare facilities.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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