Endogenous control of inflammatory visceral pain by T cell-derived opioids in IL-10-deficient mice.

Autor: Basso L; IRSD, INSERM, INRA, ENVT, UPS, Université de Toulouse, Toulouse, France., Benamar M; Centre de Physiopathologie de Toulouse Purpan (CPTP), UPS, INSERM, CNRS, Université de Toulouse, Toulouse, France., Mas-Orea X; IRSD, INSERM, INRA, ENVT, UPS, Université de Toulouse, Toulouse, France., Deraison C; IRSD, INSERM, INRA, ENVT, UPS, Université de Toulouse, Toulouse, France., Blanpied C; IRSD, INSERM, INRA, ENVT, UPS, Université de Toulouse, Toulouse, France., Cenac N; IRSD, INSERM, INRA, ENVT, UPS, Université de Toulouse, Toulouse, France., Saoudi A; Centre de Physiopathologie de Toulouse Purpan (CPTP), UPS, INSERM, CNRS, Université de Toulouse, Toulouse, France., Dietrich G; IRSD, INSERM, INRA, ENVT, UPS, Université de Toulouse, Toulouse, France.
Jazyk: angličtina
Zdroj: Neurogastroenterology and motility [Neurogastroenterol Motil] 2020 Feb; Vol. 32 (2), pp. e13743. Date of Electronic Publication: 2019 Oct 07.
DOI: 10.1111/nmo.13743
Abstrakt: Background: The opioid-mediated analgesic activity of mucosal CD4 + T lymphocytes in colitis has been reported in immunocompetent mice so far. Here, we investigated whether CD4 + T lymphocytes alleviate from inflammation-induced abdominal pain in mice with defective immune regulation.
Methods: Endogenous control of visceral pain by opioids locally produced in inflamed mucosa was assessed in IL-10-deficient mice.
Key Results: CD4 + T lymphocytes but not F4/80 + macrophages isolated from the lamina propria of IL-10-deficient mice with colitis express enkephalin-containing opioid peptides as assessed by cytofluorometry. Colitis in IL-10 -/- mice was not associated with abdominal pain. Intraperitoneal injection of naloxone-methiodide, a peripheral opioid receptor antagonist, induced abdominal hypersensitivity in IL-10 -/- mice with colitis.
Conclusion and Inferences: Opioid-mediated analgesic activity of mucosal T lymphocytes remains operating in IL-10 -/- mice with impaired immune regulation. The data suggest that endogenous T cell-derived opioids might reduce inflammation-induced abdominal pain in inflammatory bowel diseases associated with homozygous "loss of function mutations" in interleukin-10.
(© 2019 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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