Loss of genetic integrity in artificially aged seed lots of rice (Oryza sativa L.) and common bean (Phaseolus vulgaris L.).

Autor: Dantas AF; Laboratory of Genetic Toxicology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil. Electronic address: alissonfdantas@yahoo.com.br., Fascineli ML; Laboratory of Genetic Toxicology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil., José SCBR; Brazilian Agricultural Research Corporation (Embrapa) - Embrapa Genetic Resources & Biotechnology, Brasilia, Brazil., Pádua JG; Brazilian Agricultural Research Corporation (Embrapa) - Embrapa Genetic Resources & Biotechnology, Brasilia, Brazil., Gimenes MA; Brazilian Agricultural Research Corporation (Embrapa) - Embrapa Genetic Resources & Biotechnology, Brasilia, Brazil., Grisolia CK; Laboratory of Genetic Toxicology, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2019 Oct; Vol. 846, pp. 403080. Date of Electronic Publication: 2019 Aug 01.
DOI: 10.1016/j.mrgentox.2019.07.008
Abstrakt: Loss of genetic integrity can occur during the long-term conservation of seeds. We have studied these effects in seeds of rice (Oryza sativa L.) and common bean (Phaseolus vulgaris L.) exposed to accelerated aging (elevated temperature and moisture) conditions. Tests of first count, germination, and germination speed index were performed to measure physiological quality; cytogenetic tests and comet assay were used to evaluate genetic integrity. With aging, we observed a decrease in mitotic index and an increase in the frequency of chromosomal alterations in root cells of imbibed seeds, as well as increased DNA damage (comet assay) in dry and imbibed seed embryos of both species. The comet assay can be a useful technique for measuring genetic integrity in seed conservation programs.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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