| Autor: |
Morgan C; John Innes Centre, Norwich, UK. Chris.Morgan@jic.ac.uk., Wegel E; John Innes Centre, Norwich, UK. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2061, pp. 37-46. |
| DOI: |
10.1007/978-1-4939-9818-0_4 |
| Abstrakt: |
Arabidopsis arenosa has recently become established as a model organism for investigating how meiosis has evolved to overcome the meiotic challenges faced by newly formed autotetraploids. Here, we describe a protocol for the preparation of spread, immunolabeled prophase I chromosomes from established A. arenosa autotetraploids for imaging with three-dimensional structured illumination microscopy (3D-SIM). This technique allows us to dissect the unique synaptic behavior in A. arenosa and identify synaptic partner switch sites that are unresolvable with conventional widefield microscopy. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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