| Autor: |
Povey JF; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. J.Povey@kent.ac.uk., Saintas E; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. esaintas@icloud.com., Aderemi AV; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. walerem@yahoo.com., Rothweiler F; Institut für Medizinische Virologie, Klinikum der Goethe-Universität, 60596 Frankfurt am Main, Germany. f.rothweiler@kinderkrebsstiftung-frankfurt.de., Zehner R; Institut für Rechtsmedizin, Klinikum der Goethe-Universität, 60596 Frankfurt am Main, Germany. zehner@em.uni-frankfurt.de., Dirks WG; Leibniz-Institute Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH, 38124 Braunschweig, Germany. wdi@dsmz.de., Cinatl J Jr; Institut für Medizinische Virologie, Klinikum der Goethe-Universität, 60596 Frankfurt am Main, Germany., Racher AJ; Lonza Biologics, Slough SL1 4DX, UK. andy.racher@lonza.com., Wass MN; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. m.n.wass@kent.ac.uk., Smales CM; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. C.M.Smales@kent.ac.uk., Michaelis M; Industry Biotechnology Centre and School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK. M.Michaelis@kent.ac.uk. |
| Abstrakt: |
The use of cell lines in research can be affected by cell line misidentification. Short tandem repeat (STR) analysis is an effective method, and the gold standard, for the identification of the genetic origin of a cell line, but methods that allow the discrimination between cell lines of the same genetic origin are lacking. Here, we use intact cell MALDI-ToF mass spectrometry analysis, routinely used for the identification of bacteria in clinical diagnostic procedures, for the authentication of a set of cell lines consisting of three parental neuroblastoma cell lines (IMR-5, IMR-32 and UKF-NB-3) and eleven drug-adapted sublines. Principal component analysis (PCA) of intact-cell MALDI-ToF mass spectrometry data revealed clear differences between most, but not all, of the investigated cell lines. Mass spectrometry whole-cell fingerprints enabled the separation of IMR-32 and its clonal subline IMR-5. Sublines that had been adapted to closely related drugs, for example, the cisplatin- and oxaliplatin-resistant UKF-NB-3 sublines and the vincristine- and vinblastine-adapted IMR-5 sublines, also displayed clearly distinctive patterns. In conclusion, intact whole-cell MALDI-ToF mass spectrometry has the potential to be further developed into an authentication method for mammalian cells of a common genetic origin. |
