Spatial and Temporal Genetic Diversity of the Peach Potato Aphid Myzus persicae (Sulzer) in Tunisia.

Autor: Hlaoui A; Laboratoire de Protection des Végétaux, Institut National de Recherche Agronomique de Tunisie INRAT, Rue Hédi Karray, Ariana 2049, Tunisia. hlaoui_amen@hotmail.com.; Département Santé Végétale et Environnement, Institut National Agronomique de Tunisie INAT, Université de Carthage, 43 Avenue Charles Nicolle, Cité Mahrajène Tunis 1082, Tunisia. hlaoui_amen@hotmail.com., Boukhris-Bouhachem S; Laboratoire de Protection des Végétaux, Institut National de Recherche Agronomique de Tunisie INRAT, Rue Hédi Karray, Ariana 2049, Tunisia. bouhachems@gmail.com., Sepúlveda DA; Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Talca 3460000, Chile. dani.sepulveda.14@gmail.com.; Facultad de Ciencias Agrarias, Universidad de Talca, Talca 3460000, Chile. dani.sepulveda.14@gmail.com., Correa MCG; Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Talca 3460000, Chile. maggiecorrea@gmail.com.; Institut National de la Recherche Agronomique INRA, CNRS, ISA, Université Côte d'Azur, 06903 Sophia Antipolis, France. maggiecorrea@gmail.com., Briones LM; Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Talca 3460000, Chile. lbriones@utalca.cl.; Instituto de Ciencias Biológicas, Universidad de Talca, Talca 3460000, Chile. lbriones@utalca.cl., Souissi R; Laboratoire de Protection des Végétaux, Institut National de Recherche Agronomique de Tunisie INRAT, Rue Hédi Karray, Ariana 2049, Tunisia. srebha@yahoo.com., Figueroa CC; Centre for Molecular and Functional Ecology in Agroecosystems, Universidad de Talca, Talca 3460000, Chile. alfigueroa@utalca.cl.; Instituto de Ciencias Biológicas, Universidad de Talca, Talca 3460000, Chile. alfigueroa@utalca.cl.
Jazyk: angličtina
Zdroj: Insects [Insects] 2019 Oct 01; Vol. 10 (10). Date of Electronic Publication: 2019 Oct 01.
DOI: 10.3390/insects10100330
Abstrakt: The peach potato aphid, Myzus persicae (Sulzer), is a worldwide pest of many crops, and the most important aphid pest of peach and potato crops in Tunisia, mainly due to virus transmission, for which insecticides are frequently applied. We studied the genetic structure of M. persicae populations in Tunisia, in order to further our understanding of the biotic and abiotic factors shaping populations and to predict their evolutionary responses to the present management practices. We monitored peach orchards and seed potato crops in different seasons and regions from 2011-2013 and in 2016 (19 populations), assessing the genetic diversity of M. persicae at six microsatellite loci. Temporal and spatial changes in the frequency and distribution of 397 genotypes in 548 sampled aphids were studied. Only 37 genotypes were found more than once (clonal amplification), as most genotypes were found only once (91.60% in peach; 88.73% in potato crops). A similarly high genetic diversity was observed in aphids sampled from peach (G/N = 0.76; Ho = 0.617) and potato (G/N = 0.70; Ho = 0.641). Only a weak genetic differentiation among populations was found, mainly between geographic locations. Clustering analysis revealed genotypes to be grouped mainly according to host plant. The availability of the primary host, high proportion of unique genotypes, high genetic diversity and lack of structuring suggest that the aphid reproduces mainly through cyclical parthenogenesis in Tunisia. On the other hand, we provide a farm-scale study that shows how easily M. persicae can colonize different areas and hosts, which may have important implications in relation to plant virus vectoring.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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