Cacao extract enriched in polyphenols prevents endocrine-metabolic disturbances in a rat model of prediabetes triggered by a sucrose rich diet.

Autor: Castro MC; CENEXA (Centro de Endocrinología Experimental y Aplicada) (UNLP-CONICET La Plata-FCM, CEAS-CICPBA), La Plata, Argentina., Villagarcía H; CENEXA (Centro de Endocrinología Experimental y Aplicada) (UNLP-CONICET La Plata-FCM, CEAS-CICPBA), La Plata, Argentina., Nazar A; CENEXA (Centro de Endocrinología Experimental y Aplicada) (UNLP-CONICET La Plata-FCM, CEAS-CICPBA), La Plata, Argentina., Arbeláez LG; CIC (Centro de Investigaciones Cardiovasculares) (UNLP-CONICET La Plata-FCM), La Plata, Argentina., Massa ML; CENEXA (Centro de Endocrinología Experimental y Aplicada) (UNLP-CONICET La Plata-FCM, CEAS-CICPBA), La Plata, Argentina., Del Zotto H; CENEXA (Centro de Endocrinología Experimental y Aplicada) (UNLP-CONICET La Plata-FCM, CEAS-CICPBA), La Plata, Argentina., Ríos JL; Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Burjassot, Spain., Schinella GR; Cátedra Farmacología Básica, Facultad de Ciencias Médicas UNLP and CICPBA, La Plata, Argentina., Francini F; CENEXA (Centro de Endocrinología Experimental y Aplicada) (UNLP-CONICET La Plata-FCM, CEAS-CICPBA), La Plata, Argentina. Electronic address: f_francini@yahoo.com.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2020 Jan 30; Vol. 247, pp. 112263. Date of Electronic Publication: 2019 Sep 30.
DOI: 10.1016/j.jep.2019.112263
Abstrakt: Ethnopharmacological Relevance: Cocoa extracts rich in polyphenols are used as potential agent for treating diabetes. Cocoa polyphenols have been proved to ameliorate important hallmarks of type-2 diabetes (T2D). They can regulate glucose levels by increasing insulin secretion, promoting β-cell proliferation and a reduction of insulin resistance. In addition, epidemiological evidence indicates that consumption of flavonoid decreases the incidence of T2D.
Aim of the Study: T2D is preceded by a prediabetic state in which the endocrine-metabolic changes described in T2D are already present. Since epidemiological evidence indicates that consumption of flavonoid decreases its incidence, we evaluated possible preventive effects of polyphenol-enriched cocoa extract on a model of prediabetes induced by sucrose.
Materials and Methods: We determined circulating parameters and insulin sensitivity indexes, liver protein carbonyl groups and reduced glutathione, liver mRNA expression levels of lipogenic enzymes, expression of different pro-inflammatory mediators, fructokinase activity and liver glycogen content. For that, radioimmunoassay, real-time polymerase chain reaction, Western blot, spectrophotometry, and immunohistochemistry were used.
Results: We demonstrated that sucrose administration triggered hypertriglyceridemia, insulin-resistance, and liver increased oxidative stress and inflammation markers compared to control rats. Additionally, we found an increase in glycogen deposit, fructokinase activity, and lipogenic genes expression (SREBP-1c, FAS and GPAT) together with a decrease in P-Akt and P-eNOS protein content (P < 0.05). Sucrose-induced insulin resistance, hepatic carbohydrate and lipid dysmetabolism, oxidative stress, and inflammation were effectively disrupted by polyphenol-enriched cocoa extract (PECE) co-administration (P < 0.05).
Conclusion: Dietary administration of cocoa flavanols may be an effective and complementary tool for preventing or reverting T2D at an early stage of its development (prediabetes).
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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