In vitro evaluation of various antimicrobials against field mycoplasma gallisepticum and mycoplasma synoviae isolates in Egypt.

Autor:	Abd El-Hamid MI; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Awad NFS; Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Hashem YM; Department of Mycoplasma Research, Animal Health Research Institute, Giza 12622, Egypt., Abdel-Rahman MA; Department of Bacteriology, Animal Health Research Institute, Mansoura branch 35511, Egypt., Abdelaziz AM; Veterinary Diagnostic Lab. Ministry of Environment, Water &Agriculture, KSA; Veterinary Education Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Mohammed IAA; Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Abo-Shama UH; Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2019 Dec 01; Vol. 98 (12), pp. 6281-6288.
DOI:	10.3382/ps/pez576
Abstrakt:	Among many avian mycoplasmas, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are recognized as the main etiological agents of respiratory diseases and infectious synovitis in chickens and turkeys causing tremendous economic losses worldwide. Therefore, proper treatment is promoted for the control of these diseases. This study was the first in Egypt to evaluate the in vitro efficacy of various antimicrobials against field MG and MS isolates recovered from chicken and turkey flocks using both conventional broth microdilution and quantitative real-time polymerase chain reaction assays. Totally, 47 mycoplasma isolates were recovered from 160 collected tracheal samples (29.4%). Of these, 44 MG (27.5%) and 3 MS (1.9%) were identified using conventional and molecular assays. The in vitro susceptibilities of 4 representative mycoplasma field isolates (3 MG and one MS) to 8 antibiotics and 4 essential oils were investigated. The tested isolates showed various susceptibilities to tested antimicrobials. Toldin CRD, followed by clove, cumin, and cinnamon oils were effective against both MG and MS clinical isolates with minimum inhibitory concentration (MIC) values ranging from 0.49 to 15.63 µg/mL. Similarly, tylvalosin was the most active antibiotic against MG and MS isolates with the lowest MIC values (0.015 to 0.03 µg/mL). DNA loads of both MG mgc2 and MS vlhA genes were markedly decreased upon treatment with majority of the tested antimicrobials confirming their effectiveness as was also evaluated by conventional MIC results. In conclusion, Toldin CRD and tylvalosin were found to be the most effective antimicrobials in this study. This finding highlights the importance of using these antimicrobials in controlling mycoplasma infections in chickens and turkeys. (© 2019 Poultry Science Association Inc.)
Databáze:	MEDLINE
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