Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.

Autor: Qiu JJ; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.; Obstetrics and Gynecology Department of Shanghai Medical College, Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China., Lin XJ; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.; Obstetrics and Gynecology Department of Shanghai Medical College, Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China., Tang XY; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.; Obstetrics and Gynecology Department of Shanghai Medical College, Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China., Zheng TT; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.; Obstetrics and Gynecology Department of Shanghai Medical College, Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China., Zhang XY; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.; Obstetrics and Gynecology Department of Shanghai Medical College, Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China., Hua KQ; Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.; Obstetrics and Gynecology Department of Shanghai Medical College, Fudan University, Shanghai, China.; Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai, China.
Jazyk: angličtina
Zdroj: International journal of cancer [Int J Cancer] 2020 May 01; Vol. 146 (9), pp. 2588-2598. Date of Electronic Publication: 2019 Dec 05.
DOI: 10.1002/ijc.32692
Abstrakt: Peritoneal metastasis is a critical feature and clinical challenge in epithelial ovarian cancer (EOC). We previously identified a novel long noncoding RNA (lncRNA, TC0101441) in epithelial ovarian cancer (EOC) using microarrays. However, the impact of TC0101441 on EOC metastasis and prognosis remains unclear. TC0101441 expression in EOC tissues and its correlation with clinicopathological factors and prognosis were examined. A series of in vitro and in vivo assays were performed to elucidate the roles and mechanism of TC0101441 in EOC metastasis. We found that TC0101441 levels were elevated in EOC tissues compared with those in normal controls and significantly correlated with an advanced clinical stage and lymph node metastasis. TC0101441 was determined to be an independent prognostic predictor of overall survival (OS) and disease-free survival (DFS). Furthermore, loss-of-function assays showed that TC0101441 promoted the invasive and metastatic capacities of EOC cells both in vitro and in vivo. Mechanistically, the prometastatic effects of TC0101441 were linked to the induction of epithelial-mesenchymal transition (EMT). Importantly, KiSS1 was identified as a downstream target gene of TC0101441 and was downregulated by TC0101441 in EOC cells. After TC0101441 was silenced, the corresponding phenotypes of EOC cell invasion and EMT were reversed by the overexpression of KiSS1. Taken together, our data suggest that TC0101441 functions as a potential promigratory/invasive oncogene by promoting EMT and metastasis in EOC through downregulation of KiSS1, which may represent a novel prognostic marker and therapeutic target in EOC.
(© 2019 UICC.)
Databáze: MEDLINE
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