Metabolome and microbiome profiling of a stress-sensitive rat model of gut-brain axis dysfunction.

Autor: Bassett SA; Food Nutrition & Health, AgResearch Ltd., Grasslands Research Centre, Tennent Drive, Palmerston North, 4442, New Zealand.; Riddet Institute, Massey University, Palmerston North, New Zealand., Young W; Food Nutrition & Health, AgResearch Ltd., Grasslands Research Centre, Tennent Drive, Palmerston North, 4442, New Zealand.; Riddet Institute, Massey University, Palmerston North, New Zealand.; High-Value Nutrition National Science Challenge, Auckland, New Zealand., Fraser K; Food Nutrition & Health, AgResearch Ltd., Grasslands Research Centre, Tennent Drive, Palmerston North, 4442, New Zealand.; Riddet Institute, Massey University, Palmerston North, New Zealand.; High-Value Nutrition National Science Challenge, Auckland, New Zealand., Dalziel JE; Food Nutrition & Health, AgResearch Ltd., Grasslands Research Centre, Tennent Drive, Palmerston North, 4442, New Zealand. julie.dalziel@agresearch.co.nz.; Riddet Institute, Massey University, Palmerston North, New Zealand. julie.dalziel@agresearch.co.nz., Webster J; Farm Systems North, AgResearch Ltd., Ruakura Research Centre, Hamilton, New Zealand., Ryan L; Food Nutrition & Health, AgResearch Ltd., Grasslands Research Centre, Tennent Drive, Palmerston North, 4442, New Zealand., Fitzgerald P; Laboratory of Neurogastroenterology, APC Microbiome Ireland, University College Cork, Cork, Ireland., Stanton C; Department of Psychiatry and Neurobehavioural Science, University College Cork, Cork, Ireland.; Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland., Dinan TG; Laboratory of Neurogastroenterology, APC Microbiome Ireland, University College Cork, Cork, Ireland.; Department of Psychiatry and Neurobehavioural Science, University College Cork, Cork, Ireland., Cryan JF; Laboratory of Neurogastroenterology, APC Microbiome Ireland, University College Cork, Cork, Ireland.; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland., Clarke G; Laboratory of Neurogastroenterology, APC Microbiome Ireland, University College Cork, Cork, Ireland.; Department of Psychiatry and Neurobehavioural Science, University College Cork, Cork, Ireland., Hyland N; Laboratory of Neurogastroenterology, APC Microbiome Ireland, University College Cork, Cork, Ireland.; Department of Physiology, University College Cork, Cork, Ireland., Roy NC; Food Nutrition & Health, AgResearch Ltd., Grasslands Research Centre, Tennent Drive, Palmerston North, 4442, New Zealand.; Riddet Institute, Massey University, Palmerston North, New Zealand.; High-Value Nutrition National Science Challenge, Auckland, New Zealand.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Oct 01; Vol. 9 (1), pp. 14026. Date of Electronic Publication: 2019 Oct 01.
DOI: 10.1038/s41598-019-50593-3
Abstrakt: Stress negatively impacts gut and brain health. Individual differences in response to stress have been linked to genetic and environmental factors and more recently, a role for the gut microbiota in the regulation of stress-related changes has been demonstrated. However, the mechanisms by which these factors influence each other are poorly understood, and there are currently no established robust biomarkers of stress susceptibility. To determine the metabolic and microbial signatures underpinning physiological stress responses, we compared stress-sensitive Wistar Kyoto (WKY) rats to the normo-anxious Sprague Dawley (SD) strain. Here we report that acute stress-induced strain-specific changes in brain lipid metabolites were a prominent feature in WKY rats. The relative abundance of Lactococcus correlated with the relative proportions of many brain lipids. In contrast, plasma lipids were significantly elevated in response to stress in SD rats, but not in WKY rats. Supporting these findings, we found that the greatest difference between the SD and WKY microbiomes were the predicted relative abundance of microbial genes involved in lipid and energy metabolism. Our results provide potential insights for developing novel biomarkers of stress vulnerability, some of which appear genotype specific.
Databáze: MEDLINE
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