D-Alanine Is Reduced by Ocular Hypertension in the Rat Retina.

Autor: Kanamoto T; Department of Ophthalmology, Hiroshima Prefectural Hospital, Hiroshima, Japan., Sakaue H; Department of Biochemistry, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan., Kitaoka Y; Department of Ophthalmology, St. Marianna University School of Medicine, Kanagawa, Japan., Asaoka R; Department of Ophthalmology, University of Tokyo Graduate School of Medicine, Tokyo, Japan., Tobiume K; Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan., Kiuchi Y; Department of Ophthalmology and Visual Sciences, Hiroshima University, Hiroshima, Japan.
Jazyk: angličtina
Zdroj: Current eye research [Curr Eye Res] 2020 Apr; Vol. 45 (4), pp. 490-495. Date of Electronic Publication: 2019 Oct 04.
DOI: 10.1080/02713683.2019.1666995
Abstrakt: Purpose : The purpose of this study was to investigate the physiological changes of amino acids in the rat retina caused by ocular hypertension. Methods : Adult Wistar rats were used as an experimental model of ocular hypertension. Retinas were hydrolyzed with HCl at 108°C to isolate amino acids. Residual amino acids were measured by reverse-phase high-performance liquid chromatography and the total volume of residual amino acids and the ratio of D- and L-amino acids were analyzed. Free D- and L-alanine levels were also measured using two-dimensional HPLC. Results : The amount of retinal alanine decreased in ocular hypertension compared with the control ( p < .05, Student's t -test); the amounts of other amino acids did not differ between the two conditions.The D/L ratio of alanine was higher than that of other amino acids. Ocular hypertension reduced the D/L ratio of retinal alanine, while that of other amino acids was unchanged. Ocular hypertension increased the D/L ratio of free alanine. Conclusions : Ocular hypertension reduced the D/L ratio of retinal alanine, presumably due in large part to alanine peptides.
Databáze: MEDLINE
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