Improvement of the anti-proliferative activity of the peptide ERα17p in MCF-7 breast cancer cells using nanodiamonds.

Autor:	Yip F; Laboratoire des biomolécules (LBM), CNRS - UMR 7203, Sorbonne université, École normale supérieure, PSL Research University, 75252 Paris cedex 05, France., Nemati F; Laboratoire d'investigation préclinique (LIP), département de recherche translationnelle, Institut Curie, PSL Research University, 75005 Paris, France., El Botty R; Laboratoire d'investigation préclinique (LIP), département de recherche translationnelle, Institut Curie, PSL Research University, 75005 Paris, France., Belnou M; Laboratoire des biomolécules (LBM), CNRS - UMR 7203, Sorbonne université, École normale supérieure, PSL Research University, 75252 Paris cedex 05, France., Decaudin D; Laboratoire d'investigation préclinique (LIP), département de recherche translationnelle, Institut Curie, PSL Research University, 75005 Paris, France; Department of Medical Oncology, Institut Curie, 75005 Paris, France., Mansuy C; Laboratoire des biomolécules (LBM), CNRS - UMR 7203, Sorbonne université, École normale supérieure, PSL Research University, 75252 Paris cedex 05, France. Electronic address: christelle.mansuy@sorbonne.universite.fr., Jacquot Y; Laboratoire des biomolécules (LBM), CNRS - UMR 7203, Sorbonne université, École normale supérieure, PSL Research University, 75252 Paris cedex 05, France. Electronic address: yves.jacquot@sorbonne-universite.fr.
Jazyk:	angličtina
Zdroj:	Annales pharmaceutiques francaises [Ann Pharm Fr] 2019 Nov; Vol. 77 (6), pp. 488-495. Date of Electronic Publication: 2019 Sep 25.
DOI:	10.1016/j.pharma.2019.07.003
Abstrakt:	Nanodiamonds (NDs) are emerging delivery systems with biomedical applications and interesting perspectives in oncology. Their use has been proposed to assist the internalization of anticancer drugs and to decrease administered drug doses. The pro-apoptotic peptide ERα17p, which is issued from the hinge/N-terminus parts of the AF2 region of the human estrogen receptor α (ERα), is active at a concentration of 10μM on breast cancer cells and particularly on those cancer cells that are ERα-positive. We have synthesized ND@ERα17p conjugates by physisorption of the cationic peptide ERα17p on the surface of anionic NDs. Resulting ND@ERα17p suspensions were characterized by far-UV electronic circular dichroism (ECD), dynamic light scattering (DLS) and zetametry. We then tested the anti-proliferative action of ND@ERα17p on ERα-positive MCF-7 breast carcinoma cells. ND@ERα17p allowed a decrease of the active concentration to 0.1nM (ND@ERα17p), revealing unambiguously that NDs could be used to improve the anti-proliferative action of this peptide. This preliminary study proposes a novel approach for enhancing the apoptotic action displayed by ERα17p, in the context of breast cancer. (Copyright © 2019 Académie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.)
Databáze:	MEDLINE
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