Immunization with proline rich region of pneumococcal surface protein A has no role in protection against Streptococcus pneumoniae serotype 19F.

Autor: Girgis MM; Department of Microbiology & Immunology, Faculty of Pharmacy, Mansoura University, Egypt., Abd El-Aziz AM; Department of Microbiology & Immunology, Faculty of Pharmacy, Mansoura University, Egypt., Hassan R; Department of Microbiology & Immunology, Faculty of Pharmacy, Mansoura University, Egypt., Ali YM; Department of Microbiology & Immunology, Faculty of Pharmacy, Mansoura University, Egypt; Department of Veterinary Medicine, School of Biological Sciences, University of Cambridge, Cambridge, United Kingdom. Electronic address: m_youssif@mans.edu.eg.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2020 Jan; Vol. 138, pp. 103761. Date of Electronic Publication: 2019 Sep 24.
DOI: 10.1016/j.micpath.2019.103761
Abstrakt: Pneumococcal surface protein A (PspA) is one of the major virulence factors expressed by almost all pneumococcal serotypes and was suggested to be a promising universal vaccine candidate for all pneumococcal sero-groups. Here, we expressed and purified the proline-rich region (PR) of PspA and tested it as a recombinant vaccine against infection caused by a clinical isolate (SP 19 ) of Streptococcus pneumoniae serotype 19F. Our results showed that BALB/c mice immunized with recombinant proline-rich (rPR) region showed a significant higher antibody titre against rPR region compared to control non-immunized group. However, immunized mice or mice recived polyclonal antibodies against rPR region challenged via the intra-peritoneal route with a lethal dose of SP 19 isolate showed no significant difference in survival compared to control non-immunized group. These results suggested that, immunization of BALB/c mice with rPR region of PspA is not protective against infection caused by serotype 19F in a mouse model.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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