| Autor: |
Miotto YE; PPGBM - Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil., Tesser da Costa C; PPGBM - Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.; PPGBOT - Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil., de Oliveira BH; PPGBCM - Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil., Guzman F; PPGBCM - Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil., Margis R; PPGBCM - Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil., de Almeida RMC; Instituto de Física and Instituto Nacional de Ciência e Tecnologia: Sistemas Complexos, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.; Programa de Pós Graduação em Bioinformática, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil., Offringa R; Plant Developmental Genetics, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands., Dos Santos Maraschin F; PPGBM - Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil. felipe.maraschin@ufrgs.br.; PPGBOT - Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil. felipe.maraschin@ufrgs.br.; Departamento de Botânica, Universidade Federal do Rio Grande do Sul - UFRGS, Av. Bento Gonçalves 9500, Prédio 43.423, sala 216, Porto Alegre, RS, CEP 91501-970, Brazil. felipe.maraschin@ufrgs.br. |
| Abstrakt: |
Key Message: The transcriptional profile of roots is highly affected by shoot illumination. Transcriptogram analysis allows the identification of cellular processes that are not detected by DESeq. Light is a key environmental factor regulating plant growth and development. Arabidopsis thaliana seedlings grown under light display a photomorphogenic development pattern, showing short hypocotyl and long roots. On the other hand, when grown in darkness, they display skotomorphogenic development, with long hypocotyls and short roots. Although many signals from shoots might be important for triggering root growth, the early transcriptional responses that stimulate primary root elongation are still unknown. Here, we aimed to investigate which genes are involved in the early photomorphogenic root development of dark grown roots. We found that 1616 genes 4 days after germination (days-old), and 3920 genes 7 days-old were differently expressed in roots when the shoot was exposed to light. Of these genes, 979 were up regulated in 4 days and 2784 at 7 days-old. We compared the functional categorization of differentially regulated processes by two methods: GO term enrichment and transcriptogram analysis. Expression analysis of nine selected candidate genes in roots confirmed the data observed in the RNA-seq analysis. Loss-of-function mutants of these selected differentially expressed genes suggest the involvement of these genes in root development in response to shoot illumination. Our findings are consistent with the observation that dark grown roots respond to the shoot-perceived aboveground light environment. |
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