A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation.

Autor: Li L; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong. ecmtse@hku.hk yingli0e@hku.hk., Liang J; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong. ecmtse@hku.hk yingli0e@hku.hk., Luo H; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong. ecmtse@hku.hk yingli0e@hku.hk., Tam KM; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong. ecmtse@hku.hk yingli0e@hku.hk., Tse ECM; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong. ecmtse@hku.hk yingli0e@hku.hk., Li Y; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, Hong Kong. ecmtse@hku.hk yingli0e@hku.hk.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2019 Oct 10; Vol. 55 (82), pp. 12340-12343.
DOI: 10.1039/c9cc06251c
Abstrakt: A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to verify the desirable spatial resolution through enriching chromatin-associated RNAs and proteins.
Databáze: MEDLINE
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