Memory impairments and increased GFAP expression in hippocampal astrocytes following hypercaloric diet in rats.

Autor:	Bondan EF; Universidade Paulista, Programa de Pós-Graduação em Patologia Ambiental e Experimental, São Paulo SP, Brasil.; Universidade Cruzeiro do Sul, Escola de Medicina Veterinária, São Paulo SP, Brasil., Cardoso CV; Universidade Paulista, Programa de Pós-Graduação em Patologia Ambiental e Experimental, São Paulo SP, Brasil., Martins MFM; Universidade Cruzeiro do Sul, Escola de Medicina Veterinária, São Paulo SP, Brasil., Otton R; Universidade Cruzeiro do Sul, Programa de Pós-Graduação em Ciências da Saúde, São Paulo SP, Brasil.
Jazyk:	angličtina
Zdroj:	Arquivos de neuro-psiquiatria [Arq Neuropsiquiatr] 2019 Sep 23; Vol. 77 (9), pp. 601-608. Date of Electronic Publication: 2019 Sep 23 (Print Publication: 2019).
DOI:	10.1590/0004-282X20190091
Abstrakt:	Objective: Hypothalamic inflammation and glial fibrillary acidic protein (GFAP) overexpression in astrocytes are well described in obese animals, as are some cognitive and memory deficits. As the hippocampus plays important roles in the consolidation of information, this investigation aimed to observe the memory function and the astrocyte expression of GFAP in the hippocampus of rats that received either a hypercaloric or a normocaloric diet. Methods: Adult male Wistar rats received a high-fat (cafeteria) or a standard diet for 60 days. On the 61st day, the rats were submitted to the novel object recognition (NOR) test at three and 24 hours after the first contact with objects, to assess short-term and long-term memory, respectively. Thereafter, the rats were euthanized and their brains were collected for GFAP immunohistochemical investigation in the hippocampus (CA1, CA2, CA3 areas) and hypothalamus (periventricular and arcuate nuclei). Astrocytic reactivity was assessed by morphometry. Different white adipose tissue depots and brown adipose tissue were weighed to calculate the adiposity index. Results: The hypercaloric diet increased body weight gain, adiposity index, white adipose tissue weight (epididymal, subcutaneous and retroperitoneal) and brown adipose tissue weight. Rats fed with the hypercaloric diet showed short-term and long-term memory impairments in the NOR test, as well as increased GFAP expression in astrocytes from all analyzed hypothalamic and hippocampal areas. Conclusion: This astrogliosis suggests that the neuroinflammatory response also occurs in the hippocampus and may be involved in the memory losses observed in obese/overweight animals.
Databáze:	MEDLINE
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