| Autor: |
Bermejo-Velasco D, Kadekar S, Tavares da Costa MV, Oommen OP; Bioengineering and Nanomedicine Lab, Faculty of Medicine and Health Technologies and BioMediTech Institute , Tampere University , Korkeakoulunkatu 3 , Tampere 33720 , Finland., Gamstedt K, Hilborn J, Varghese OP |
| Jazyk: |
angličtina |
| Zdroj: |
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2019 Oct 16; Vol. 11 (41), pp. 38232-38239. Date of Electronic Publication: 2019 Oct 04. |
| DOI: |
10.1021/acsami.9b10239 |
| Abstrakt: |
Currently, there are limited approaches to tailor 3D scaffolds cross-linked with a stable covalent C-C bond that does not require any catalysts or initiators. We present here the first hydrogels employing aldol condensation chemistry that exhibit exceptional physicochemical properties. We investigated the aldol-cross-linking chemistry using two types of aldehyde-modified hyaluronic acid (HA) derivatives, namely, an enolizable HA-aldehyde (HA-Eal) and a non-enolizable HA-aldehyde (HA-Nal). Hydrogels formed using HA-Eal demonstrate inferior cross-linking efficiency (due to intramolecular loop formation), when compared with hydrogels formed by mixing HA-Eal and HA-NaI leading to a cross-aldol product. The change in mechanical properties as a result of cross-linking at different pH values is determined using rheological measurements and is interpreted in terms of molecular weight between cross-links ( M c ). The novel HA cross-aldol hydrogel demonstrate excellent hydrolytic stability and favorable mechanical properties but allow hyaluronidase-mediated enzymatic degradation. Interestingly, residual aldehyde functionality within the aldol product rendered the tissue-adhesive properties by bonding two bone tissues. The aldehyde functionality also facilitated facile post-synthetic modifications with nucleophilic reagents. Finally, we demonstrate that the novel hydrogel is biocompatible with encapsulated stem cells that show a linear rate of expansion in our 3-6 days of study. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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