Atrazine-induced toxicity in goat spermatozoa is alleviated to some extent by polyphenol-enriched feed.

Autor: Komsky-Elbaz A; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel; Animal Sperm Research Center, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel; Center of Excellence in Agriculture and Environmental Health, The Hebrew University of Jerusalem, Israel., Saktsier M; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel; Animal Sperm Research Center, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel., Biran D; Extension Services, Ministry of Agriculture and Rural Development, Bet Dagan, Israel., Argov-Argaman N; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel., Azaizeh H; The Institute of Applied Research (affiliated with University of Haifa), The Galilee Society, Shefa-Amr, 20200, Israel; Tel-Hai College, Department of Environmental Sciences, Upper Galilee, 12208, Israel., Landau YS; Department of Natural Resources and Agronomy, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel., Roth Z; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel; Animal Sperm Research Center, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot, Israel; Center of Excellence in Agriculture and Environmental Health, The Hebrew University of Jerusalem, Israel. Electronic address: z.roth@mail.huji.ac.il.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2019 Dec; Vol. 236, pp. 124858. Date of Electronic Publication: 2019 Sep 14.
DOI: 10.1016/j.chemosphere.2019.124858
Abstrakt: Atrazine (ATZ) is one of the most extensively used herbicides to control growth of broadleaf and grassy weeds in crops. ATZ and its metabolites have deleterious effect on sperm quality. ATZ is also known for its ability to induce oxidative stress. Pistacia lentiscus (PL) is an evergreen shrub, with a high content of polyphenols in leaf extracts, with a known anti-inflammatory and antioxidant properties. The protective effect of PL or its extracts against ATZ-induced damage have not been yet evaluated. We examined the harmful effects of atrazine (ATZ) exposure on male reproductive system, using goat (Capra hircus) model spermatozoa and the protective effects of PL and PL ethanolic extract (PLE). In in-vivo experiments, male goats were fed a standard ration or one supplemented with 15 mg ATZ/kg body weight daily, for 6 months. Exposure to ATZ impaired the spermatozoa's morphology, viability, mitochondrial membrane potential and cell lipid composition. These alterations may in turn lead to reduced fertilization competence of the exposed spermatozoa. In an ex-vivo experiment, spermatozoa from male goats fed a standard ration or one supplemented with PL or PLE for 90 days and then were exposed to 1 μM ATZ or 10 μM of its major metabolite diaminochlorotriazine (DACT) through in-vitro capacitation. Prefeeding with PL or PLE partially attenuated the harmful effects of ATZ and DACT. Dietary supplementation with polyphenol-enriched feed can protect, to a certain extent, spermatozoa in males exposed to environmental toxicants.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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