Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation.

Autor:	Ben Mefteh F; NextBiotech, 98 Rue Ali Belhouane, Agareb 3030, Tunisia. fedia.benmefteh@gmail.com.; Faculty of Sciences, University of Sfax, Sfax 3029, Tunisia. fedia.benmefteh@gmail.com., Bouket AC; Plant Protection Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Tabriz 5355179854, Iran. a.chenari@areeo.ac.ir., Daoud A; NextBiotech, 98 Rue Ali Belhouane, Agareb 3030, Tunisia. amal.daoud42@gmail.com., Luptakova L; NextBiotech, 98 Rue Ali Belhouane, Agareb 3030, Tunisia. Lenka.Luptakova@uvlf.sk.; Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, 04181 Kosice, Slovakia. Lenka.Luptakova@uvlf.sk., Alenezi FN; NextBiotech, 98 Rue Ali Belhouane, Agareb 3030, Tunisia. Dr-Faizah@outlook.com., Gharsallah N; Faculty of Sciences, University of Sfax, Sfax 3029, Tunisia. neji.gharsallah@fss.rnu.tn., Belbahri L; NextBiotech, 98 Rue Ali Belhouane, Agareb 3030, Tunisia. lassaad.belbahri@unine.ch.; Laboratory of Soil Biodiversity, University of Neuchâtel, CH-2000 Neuchatel, Switzerland. lassaad.belbahri@unine.ch.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2019 Sep 23; Vol. 7 (10). Date of Electronic Publication: 2019 Sep 23.
DOI:	10.3390/microorganisms7100382
Abstrakt:	The phosphogypsum (PG) endogenous bacterial community and endophytic bacterial communities of four plants growing in phosphogypsum-contaminated sites, Suaeda fruticosa ( SF ), Suaeda mollis ( SM ), Mesembryanthmum nodiflorum ( MN ) and Arthrocnemum indicum ( AI ) were investigated by amplicon sequencing. Results highlight a more diverse community of phosphogypsum than plants associated endophytic communities. Additionally, the bacterial culturable communities of phosphogypsum and associated plant endophytes were isolated and their plant-growth promotion capabilities, bioremediation potential and stress tolerance studied. Most of plant endophytes were endowed with plant growth-promoting (PGP) activities and phosphogypsum communities and associated plants endophytes proved highly resistant to salt, metal and antibiotic stress. They also proved very active in bioremediation of phosphogypsum and other organic and inorganic environmental pollutants. Genome sequencing of five members of the phosphogypsum endogenous community showed that they belong to the recently described species Bacillus albus ( BA ). Genome mining of BA allowed the description of pollutant degradation and stress tolerance mechanisms. Prevalence of this tool box in the core, accessory and unique genome allowed to conclude that accessory and unique genomes are critical for the dynamics of strain acquisition of bioremediation abilities. Additionally, secondary metabolites (SM) active in bioremediation such as petrobactin have been characterized. Taken together, our results reveal hidden untapped valuable bacterial actors for waste remediation. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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