Mercury resistance and bioremediation mediated by endophytic fungi.

Autor: Pietro-Souza W; Federal Institute of Education, Science and Technology of Mato Grosso, Lucas Do Rio Verde, Mato Grosso, Brazil., de Campos Pereira F; Department of Botany and Ecology, Laboratory of Biotechnology and Microbial Ecology, Institute of Biosciences, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil., Mello IS; Department of Forest Engineering, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil., Stachack FFF; Fuel Analysis Centre (CEANC), Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil., Terezo AJ; Fuel Analysis Centre (CEANC), Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil., Cunha CND; Department of Botany and Ecology, Laboratory of Biotechnology and Microbial Ecology, Institute of Biosciences, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil., White JF; Department of Plant Biology, Rutgers University, New Brunswick, NJ, USA., Li H; Medical School, Kunming University of Science and Technology, Kunming, Yunnan, China., Soares MA; Department of Botany and Ecology, Laboratory of Biotechnology and Microbial Ecology, Institute of Biosciences, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil. Electronic address: drmasoares@gmail.com.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2020 Feb; Vol. 240, pp. 124874. Date of Electronic Publication: 2019 Sep 16.
DOI: 10.1016/j.chemosphere.2019.124874
Abstrakt: The present study proposes the use of endophytic fungi for mercury bioremediation in in vitro and host-associated systems. We examined mercury resistance in 32 strains of endophytic fungi grown in culture medium supplemented with toxic metal concentrations. The residual mercury concentrations were quantified after mycelial growth. Aspergillus sp. A31, Curvularia geniculata P1, Lindgomycetaceae P87, and Westerdykella sp. P71 were selected and further tested for mercury bioremediation and bioaccumulation in vitro, as well as for growth promotion of Aeschynomene fluminensis and Zea mays in the presence or absence of the metal. Aspergillus sp. A31, C. geniculata P1, Lindgomycetaceae P87 and Westerdykella sp. P71 removed up to 100% of mercury from the culture medium in a species-dependent manner and they promoted A. fluminensis and Z. mays growth in substrates containing mercury or not (Dunnett's test, p < 0.05). Lindgomycetaceae P87 and C. geniculata P1 are dark septate endophytic fungi that endophytically colonize root cells of their host plants. The increase of host biomass correlated with the reduction of soil mercury concentration due to the metal bioaccumulation in host tissues and its possible volatilization. The soil mercury concentration was decreased by 7.69% and 57.14% in A. fluminensis plants inoculated with Lindgomycetaceae P87 + Aspergillus sp. A31 and Lindgomycetaceae P87, respectively (Dunnet's test, p < 0.05). The resistance mechanisms of mercury volatilization and bioaccumulation in plant tissues mediated by these endophytic fungi can contribute to bioremediation programs. The biochemical and genetic mechanisms involved in bioaccumulation and volatilization need to be elucidated in the future.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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